三黄泻心汤抑制α-淀粉酶及α-葡萄糖苷酶活性成分研究  

Study on the active components of Sanhuang Xiexin Decoction in inhibitingα-amylase andα-glucosidase activities

作　　者：方鹏霞 赵磊 段文达 武晓玉 续艳丽 张启立 王洁 禄国花 FANG Peng-xia;ZHAO Lei;DUAN Wen-da;WU Xiao-yu;XU Yan-li;ZHANG Qi-li;WANG Jie;LU Guo-Hua(School of Pharmacy,Gansu University of Chinese Medicine,Lanzhou Gansu 730000,China;Northwest Collaborative Innoution Center for Traditional Chinese Medicine(TCM),Lanzhou Gansu 730000,China;Key Laboratory of Chemistry and Quality for TCM of the College of Gansu Province,Lanzhou Gansu 730000,China;Gansu Province Engineering Laboratory for TCM Standardization Technology and Popularisation,Lanzhou Gansu,730000 China;Gansu Pharmaceutical Industry Innoration Research Institute,Lanzhou Gansu,730000,China;lanzhou Institute of Food and Drug Control,Lanzhou,Gansu 730000,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]西北中藏药省部共建协同创新中心,甘肃兰州730000 [3]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000 [4]甘肃省道地药材质量标准化研究与推广工程实验室,甘肃兰州730000 [5]陇药产业创新研究院,甘肃兰州730000 [6]兰州市食品药品检验检测研究院,甘肃兰州730000

出　　处：《时珍国医国药》2024年第4期795-798,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(82160457);甘肃省科技计划资助项目(21JR7RA564);甘肃省教育厅“双一流”科研重点项目(GSSYLXM-05);甘肃省中医药管理局(GZKG-2021-53);甘肃省高等学校青年博士基金项目(2022QB-100);甘肃省道地药材质量标准化技术研究与推广工程实验室开放基金项目(ddyc-2022-01)。

摘　　要：目的筛选三黄泻心汤抑制α-淀粉酶和α-葡萄糖苷酶抑制活性的有效部位,并研究其活性成分。方法三黄泻心汤水煎液采用大孔树脂分段洗脱得到水部位、30%乙醇部位、60%乙醇部位、95%乙醇部位,对水煎液及4个洗脱部位进行α-淀粉酶和α-葡萄糖苷酶体外活性抑制试验,筛选最佳活性部位,再通过高效液相色谱分析其活性成分,并进行方法学考察与含量测定。结果筛选得到抑制α-淀粉酶活性最佳部位为30%乙醇部位,IC50为1.707mg·mL^(-1),活性成分主要有黄芩苷,小檗碱,汉黄芩苷,黄芩素,芦荟大黄素,大黄酸,大黄素,其中黄芩苷含量最高(177.588 mg·g^(-1)),汉黄芩苷次之(30.546 mg·g^(-1));抑制α-葡萄糖苷酶活性最佳部位为60%乙醇部位,IC50为0.0123mg·mL^(-1),活性成分有黄芩苷,小檗碱,汉黄芩苷,黄芩素,芦荟大黄素,大黄酸,大黄素,大黄酚,大黄素甲醚,其中黄芩苷含量最高(181.631mg·g^(-1)),小檗碱次之(71.724 mg·g^(-1))。结论三黄泻心汤在一定程度上对α-淀粉酶及α-葡萄糖苷酶均有活性抑制作用,对α-葡萄糖苷酶的抑制作用优于α-淀粉酶,对开发α-葡萄糖苷酶抑制剂具有一定参考价值。Objective To screen the effective parts of Sanhuang Xiexin Decoction for inhibiting the inhibitory activities ofα-amylase andα-glucosidase,and to study its active components.Methods The aqueous decoction of Sanhuang Xiexin Decoction was eluted with macroporous resin to obtain the aqueous part,30%ethanol part,60%ethanol part and 95%ethanol part,and the aqueous decoction and the four eluted parts were subjected toα-amylase andα-glucosidase inhibition tests in vitro to screen the best active parts,and then analyzed the active components by high performance liquid chromatography,and the methodological investigations and content determinations were carried out.Results The best site for inhibitingα-amylase activity was screened to be the 30%ethanol site,with an IC_(50)of 1.707 mg·mL^(-1),and the active ingredients were baicalin,berberine,wogonoside,baicalein,aloe-emodin,rhein and emodin,with baicalin being the most abundant(177.588 mg·g^(-1)),and wogonoside being the second most abundant(30.546 mg·g^(-1));The best site for inhibition ofα-glucosidase activity was the 60%ethanol site with an IC_(50)of 0.0123 mg·mL^(-1),and the active ingredients were baicalin,berberine,wogonoside,baicalein,aloe-emodin,rhein,emodin,chrysophanol,and physcion,with baicalin being the most abundant(181.631 mg·g^(-1)),and berberine being second most abundant(71.724 mg·g^(-1)).Conclusion Sanhuang Xiexin Decoction has active inhibitory effect on bothα-amylase andα-glucosidase to a certain extent,and the inhibitory effect onα-glucosidase is better than that onα-amylase,which is of some reference value for the development ofα-glucosidase inhibitors.

关 键 词：三黄泻心汤 Α-淀粉酶 Α-葡萄糖苷酶 抑制作用 活性成分 

分 类 号：R283[医药卫生—中药学] R284[医药卫生—中医学]