高压酸水解-离子交换色谱法测定乳粉中的酵母β-葡聚糖  

Determination of Yeast β-glucan in Milk Powder by High Pressure Acid Hydrolysis-high Performance Ion Chromatography

作　　者：李燕妹 黄燕 梁敏 蔡小明 吴丽倩 唐雯 叶梦薇 黄媛 Li Yanmei;Huang Yan;Liang Min;Cai Xiaoming;Wu Liqian;Tang Wen;Ye Mengwei;Huang Yuan(National Quality Supervision and Testing Center for Processed Food(Fuzhou),Fujian Inspection and Research Institute for Product Quality,Fuzhou 350000,China)

机构地区：[1]福建省产品质量检验研究院国家加工食品质量检验检测中心(福州),福建福州350000

出　　处：《山东化工》2024年第11期118-121,共4页Shandong Chemical Industry

基　　金：福建省产品质量检验研究院科技项目(KY202116A)。

摘　　要：目的:采用高压酸水解-离子交换色谱法建立乳粉中酵母β-葡聚糖含量的分析方法。方法:先对乳粉中的酵母β-葡聚糖进行富集,富集得到的酵母β-葡聚糖利用高压酸水解成葡萄糖后利用离子色谱法检测分析,最后通过公式换算计算出酵母β-葡聚糖的含量。结果:葡萄糖在2~200 mg/L质量浓度范围内的线性关系良好,相关系数r为0.9998。酵母β-葡聚糖的定量限为11.1 mg/100 g。在20,50,100 mg/100 g加标水平下,酵母β-葡聚糖的加标回收率为81.5%~99.8%,相对标准偏差小于5%。结论:该方法简便高效、灵敏度高、准确性好,适用于乳粉中酵母β-葡聚糖的定量检测。Objective:A method for the determination of yeast β-glucan in milk powder was developed by high pressure acid hydrolysis and ion exchange chromatography.Methods:The yeastβ-glucan in milk powder was first enriched,and then was hydrolyzed into glucose by high pressure acid,which was analyzed by ion chromatography.The content of yeastβ-glucan was calculated by formula conversion.Results:The linear relationship between the peak areas and concentration of glucose in 2~200 mg/L was good,which the correlation coefficient was 0.9998.The quantitative limit of yeast β-glucan was 11.1 mg/100 g.The recoveries of yeast β-glucan at three spiked levels were in the range of 81.5%~99.8%,and the relative standard deviations were all bellow 5%.Conclusion:The study indicated that the method was simplicity,efficiency,high sensitivity and accuracy,which was suitable for the determination of yeast β-glucan in milk powder.

关 键 词：高压酸水解 离子交换色谱法 乳粉 酵母Β-葡聚糖 

分 类 号：O657.7[理学—分析化学]