miR-557 suppresses hepatocellular carcinoma cell proliferation and migration via downregulating CBX4  

作　　者：XULONG SUN WENTAO DING CHAO JIANG ZHIAN FANG 

机构地区：[1]Department of Orthopedics,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200080,China [2]Department of General Surgery,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200080,China [3]Department of Thoracic Surgery,Shanghai Chest Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200030,China [4]Department of Radiology,Zhongda Hospital,Southeast University School of Medicine,Nanjing,210009,China

出　　处：《BIOCELL》2024年第7期1071-1079,共9页生物细胞（英文）

摘　　要：Introduction:Hepatocellular carcinoma(HCC),a prevalent malignancy,poses significant challenges with high tumor heterogeneity and poor prognosis.MicroRNAs(miRNAs)play a pivotal role in hepatocarcinogenesis.Although abnormalities in microRNA-557(miR-557)expression have been implicated in various cancer types,its role in HCC remains unclear.Therefore,there is a need to explore the function of microRNA-557 in HCC.Methods:Candidate miRNAs were identified through screening in GSE108724 and GSE20077.Real-time PCR was employed to analyze the expression level of miR-557 in hepatoma cell lines and tissues.Cell viability and migration assays were applied to assess the impact of miR-557 on HCC cell lines.Furthermore,the miR-557 target was predicted through three algorithms(Targetscan,miRWalk,and miRanda),and this was confirmed through luciferase assay and Western blotting.Results:In this study,miR-557 was identified in two datasets and expressed at a low level in both hepatoma cell lines and tissues.Notably,high expression of miR-557 in HCC cells inhibited oncogenesis.Conversely,low expression of miR-557 enhanced tumor proliferation and migration.Polycomb chromobox 4(CBX4)was identified as a direct target of miR-557.Silencing CBX4 influenced the functional impact of miR-557 on HCC cell migration.Conclusion:Taken together,our study contributed to elucidating the hepatoma molecular heterogeneity and provided novel insights into miR-557 role and its target CBX4 in HCC,suggesting its potential as a future effectively druggable target for HCC intervention.

关 键 词：Hepatocellular carcinoma miR-557 Chromobox 4 HEPATOCARCINOGENESIS 

分 类 号：R735.7[医药卫生—肿瘤]