不同干燥方式八角果实挥发性化合物代谢组分析  

Metabolomic Analysis on Volatile Compounds from Illicium verum Fruits with Different Drying Methods

作　　者：李玉祥[1] 黄兴粉 王灿 滕娟[1] 杨玉玲 王晓静 孙宏伟 LI Yuxiang;HUANG Xingfen;WANG Can;TENG Juan;YANG Yuling;WANG Xiaojing;SUN Hongwei(Wenshan Academy of Agricultural Sciences,Wenshan,Yunnan 663099,China)

机构地区：[1]文山州农业科学院,云南文山663099

出　　处：《贵州农业科学》2024年第6期1-12,共12页Guizhou Agricultural Sciences

基　　金：文山州创新人才项目(KJJSCX-2020004);八角高质量发展项目([2021]26号)。

摘　　要：【目的】探明不同干燥方式对八角果实挥发性成分的影响,筛选出适宜八角果实干燥的处理方式,为八角后期深加工、保存及应用提供参考。【方法】以新鲜八角果实为材料,采用自然晾晒、40℃、50℃、60℃、70℃、80℃和90℃烘干7种处理方式进行干燥,通过液相色谱-质谱(LC-MS)技术检测分析八角果实代谢产物,阐明八角果实不同温度干燥与自然晾晒处理的代谢物差异。【结果】与自然晾晒相比,八角果实40℃干燥差异代谢产物有11类17种,其中,萜类、醇类和醛类3类代谢物上调;50℃干燥差异代谢物有10类25种,其中,上调代谢物6类(萜类、醇类、酯类、酮类、卤代烃类、酚类);60℃干燥差异代谢物有8类13种,其中,醇类和烃类代谢物上调;70℃干燥差异代谢物为13类40种,其中,醇类、萜类和酯类3类代谢物上调;80℃干燥差异代谢物有13类38种,仅醇类代谢物上调;90℃干燥差异代谢物有13类52种,其中,上调代谢物4类(萜类、醇类、酮类、卤代烃类)。40℃、50℃和60℃干燥的八角果实与自然晾晒差异代谢物较少,上调代谢物主要是苯甲醛、醋酸植物醇、茴香脑、6-异羟基樟脑、乙酸异丁香酚酯和甲基丁香酚等挥发性化合物,提升了八角浓厚的香味。70℃、80℃和90℃干燥的八角果实与自然晾晒差异代谢物较多,且与八角香味相关的许多代谢物下调表达,主要有香芹酮、茴香脑、E-氧化玫瑰、4-蒈烯、对薄荷脑1,5,8-三烯、苯甲醛、芳樟醇、3-甲基苯并噻吩、2-甲基-2-四氢呋喃和2-乙酰基-5-甲基呋喃等挥发性化合物,减弱了八角香味。【结论】不同干燥处理八角果实的挥发性化合物种类均存在一定差异。40℃、50℃和60℃干燥八角果实,苯甲醛、醋酸植物醇和茴香脑等上调表达的挥发性化合物提升了八角香味。八角果实经50℃干燥后,上调表达的代谢物有萜类、醇类、酯类、酮类、卤代烃【Objective】The effects of different drying treatments on volatile components of Illicium verum fruits were studied,which provided reference for the deep processing,preservation,and application of I.verum in the later stage.【Method】Fresh I.verum fruits were used as materials,treated with seven drying methods(natural drying,drying at 40℃,50℃,60℃,70℃,80℃and 90℃)respectively,and then the metabolite components from I.verum fruits were detected by liquid chromatograph-mass spectrometer(LC-MS),and the differences of metabolite components between the treatment of natural drying and the treatment of drying at different temperatures were clarified.【Result】Compared with natural drying,there were 17 species and 11 types of differential metabolites in I.verum fruits dried at 40℃,among which 3 types were up-regulated metabolites(terpenoid,alcohol and aldehyde).There were 25 species and 10 types of differential metabolites in I.verum fruits dried at 50℃,among which 6 types were up-regulated metabolites(terpenoid,alcohol,esters,ketones,halogenated hydrocarbon and phenol).There were 13 species and 8 types of differential metabolites in I.verum fruits dried at 60℃,among which alcohol and hydrocarbon were up-regulated metabolites.There were 40 species and 13 types of differential metabolites in I.verum fruits dried at 70℃,among which alcohol,terpenoid and esters were up-regulated metabolites.There were 38 species and 13 types of differential metabolites in I.verum fruits dried at 80℃,among which alcohol was up-regulated metabolite.There were 52 species and 13 types of differential metabolites in I.verum fruits dried at 90℃,among which 4 types were up-regulated metabolites(terpenoid,alcohol,ketones and halogenated hydrocarbon).The metabolites of I.verum fruits treated at 40℃,50℃and 60℃were less different from that of I.verum fruits treated by natural drying,and the main up-regulated metabolites were benzaldehyde,acetate plant alcohol,anethole,6-isohydroxycamphor,acetyl eugenol and methyleuge

关 键 词：八角 干燥温度 干燥 自然晾晒 液相色谱-质谱 挥发性化合物 

分 类 号：S573[农业科学—作物学] TS264[轻工技术与工程—发酵工程]