液相色谱-串联质谱法快速测定猪肉中5种激素类药物残留  

Rapid Determination of Residues of Five Hormone Drugs in Pork by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：周源槟 叶小霞 ZHOU Yuanbing;YE Xiaoxia(Shenzhen Sanfangyuan Inspection Regulatory Service Co.,Ltd.,Shenzhen 518110,China)

机构地区：[1]深圳三方圆检测监管服务有限公司,广东深圳518110

出　　处：《食品安全导刊》2024年第11期126-129,共4页China Food Safety Magazine

摘　　要：本文建立液相色谱-串联质谱法快速测定猪肉中睾酮、甲睾酮、群勃龙、诺龙及丙酸睾酮的方法。样品经叔丁基甲醚均质、振荡提取,旋转蒸发浓缩后用50%乙腈水溶液复溶,冷冻离心脱脂净化,过膜后供液质联用仪测定,内标法定量。结果表明,5种激素类药物在2~100 ng·mL^(-1)线性关系良好,定量限均为1.0 μg·kg^(-1),加标回收率为66.2%~118.0%,相对标准偏差为1.1%~7.1%。这表明液相色谱-串联质谱法适用于猪肉中5种激素类药物残留的定性定量检测。The method for rapid determination of testosterone,methyltestosterone,trenbolone,nandrolone and testosterone propionate in pork by liquid chromatography-tandem mass spectrometry was established.The samples were homogenized and extracted by shock,then redissolved with 50%acetonitrile solution by rotating evaporation,purified by freezing centrifuge,then determined by liquid chromatograph/mass spectrometer instrument after passing the membrane and quantified by internal standard method.The results showed that the five hormone components had good linear relationships in the range of 2~100 ng·mL^(-1),the limits of quantification were 1.0μg·kg^(-1),the recoveries were 66.2%~118.0%,and the relative standard deviation were 1.1%~7.1%.This indicated that liquid chromatography-tandem mass spectrometry was suitable for the qualitative and quantitative determination of five hormone drug residues in pork.

关 键 词：激素类药物 猪肉 液相色谱-串联质谱法 药物残留 

分 类 号：O657.63[理学—分析化学] TS251.51[理学—化学]