高效液相色谱法测定酱卤肉中11种磺酸基团着色剂  

Determination of 11 kinds of sulfonic acid group colorants in sauced meat by high performance liquid chromatography

作　　者：杨芮 范钦杰 卢帮贵 彭明军 邓迎春 刘颖 YANG Rui;FAN Qin-jie;LU Bang-gui;PENG Ming-jun;DENG Ying-chun;LIU Ying(Henan Institute of Food and Salt Industry Inspection Technology,Zhengzhou 450003,Henan,China)

机构地区：[1]河南省食品和盐业检验技术研究院,河南郑州450003

出　　处：《粮食与油脂》2024年第7期133-137,共5页Cereals & Oils

基　　金：河南省市场监督管理局科技计划项目(2023sj97)。

摘　　要：建立通过高效液相色谱法检测酱卤肉中11种磺酸基团着色剂的方法。样品经提取剂(无水乙醇∶乙腈∶氨水∶水体积比50∶20∶20∶10)提取,调节上样溶液pH 2沉淀蛋白质后,过混合弱阴离子固相萃取柱净化。采用20 mmol/L乙酸铵(A)-甲醇(B)作为流动相进行梯度洗脱,采用光电二级阵列管检测器(PDA)对11种磺酸基团着色剂进行多波长检测。结果表明:方法的回收率为60.8%~96.2%,相对标准偏差均小于15%(n=6),检出限均可达到0.5 mg/kg。建立的方法可对11种磺酸基团着色剂进行同时检测。A method for determination 11 kinds of sulfonic acid group colorants in sauced meat was established by high performance liquid chromatography.The sample was extracted by extraction agent(anhydrous ethanol∶acetonitrile∶ammonia∶water volume ratio 50∶20∶10∶10),and the protein was precipitated by adjusting the pH of the sample solution to 2.The mixture was purified by a weak anion solidphase extraction column.20 mmol/L ammonium acetate(A)-methanol(B)was used as mobile phases for gradient elution,and a photoelectric secondary array tube detector(PDA)was used for multi wavelength detection of 11 kinds of sulfonic acid group colorants.The results showed that the recovery rate of the method was 60.8%-96.2%,the relative standard deviation was less than 15%(n=6),and the detection limits could reach 0.5 mg/kg.The method established could be used for simultaneous detection of 11 kinds of sulfonic acid group colorants.

关 键 词：酸性橙Ⅱ 高效液相色谱 酱卤肉 着色剂 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]