刺梨多酚提取物对高脂饮食诱导的肥胖小鼠的改善作用  

Ameliorating Effect of Polyphenol Extract of Rosa roxburghii on Obese Mice Induced by High Fat Diet

作　　者：罗婧文 张欣 王辉 龙明秀 陈朝军 陈中爱 王梅 LUO Jingwen;ZHANG Xin;WANG Hui;LONG Mingxiu;CHEN Zhaojun;CHEN Zhong'ai;WANG Mei(Institute of Food Processing Technology,Guizhou Academy of Agricultural Sciences,Guiyang 550025,China;Guizhou Key Laboratory of Agricultural Biotechnology,Guiyang 550025,China;School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China;Insititute of Integrated Agricultural Development,Guiyang 550025,China)

机构地区：[1]贵州省农科院食品加工研究所,贵州贵阳550025 [2]贵州省农业生物技术重点实验室,贵州贵阳550025 [3]贵州大学酿酒与食品工程学院,贵州贵阳550025 [4]贵州省现代农业发展研究所,贵州贵阳550025

出　　处：《食品工业科技》2024年第14期352-360,共9页Science and Technology of Food Industry

基　　金：贵州省科技厅科技支撑项目(黔科合支撑[2022]一般149;黔科合支撑[2021]一般116;黔科合支撑[2020]1Y155号);贵州省科技厅2023年度贵州省基础研究计划(黔科合基础-ZK[2023]一般182)。

摘　　要：目的:为了研究刺梨多酚提取物对高脂饮食诱导的肥胖小鼠的改善作用。方法:分别以多酚含量400 mg/kg BW/d的剂量对高脂饮食小鼠进行干预。连续灌胃4周,测定小鼠体重、肝脏指数、白色脂肪及肝脏组织形态学变化、血清生化指标、脏器抗氧化能力和肠道屏障。结果:与CON组相比,MODEL组胰岛素水平(Insulln,INS)和炎症因子水平均显著(P<0.05,P<0.01)提高,血脂四项中高密度脂肪(High-density lipoprotein-cholesterol,HDL-C)水平显著降低(P<0.01),总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)和低密度脂肪(Low-density lipoprolesterol,LDL-C)水平显著升高(P<0.01);抗氧化物中超氧化物歧化酶(Superoxide Dismutase,SOD)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GPx)活力显著下降(P<0.01),肠道屏障蛋白表达水平及短链脂肪酸(Short-chain fatty acids,SCFAs)水平均显著降低(P<0.01);与MODEL相比,多酚干预组(RF、RP组)炎症因子和氧化应激水平显著降低(P<0.05,P<0.01),RF组抗氧化物SOD、GPx活力显著提升(P<0.01),RF组和RP组肠道内ZO-1、Claduin-1和SCFAs水平均发生显著逆转(P<0.01)。结论:刺梨多酚提取物能有效缓解高脂饮食诱导的肥胖小鼠体内代谢紊乱,减缓体重增长,保护脂肪和肝脏组织细胞形态变化,缓解肥胖小鼠体内炎症和氧化应激水平,增强肠道屏障功能,且RF效果优于RP。Objective:To investigate the beneficial effects of phenolic extracts of Rosa roxburghii Tratt.on obese mice induced by a high-fat diet.Methods:The intervention involved administering polyphenol extract at a dose of 400 mg/kg body weight per day to mice on a high-fat diet.The mice were orally gavaged continuously for 4 weeks,and various parameters such as body weight,liver index,histomorphological changes in white fat and liver tissues,serum biochemical markers,organ antioxidant capacity,and intestinal barrier function were examined.Results:In comparison to control group(CON),the MODEL group(MODEL)exhibited significantly(P<0.05,P<0.01)increased the level of insulin and inflammatory factors.The levels of high-density lipoprotein cholesterol(HDL-C)decreased in the four blood lipids,while the levels of total cholesterol(TC),triglycerides(TG),and low-density lipoprotein cholesterol(LDL-C)significantly increased(P<0.01).The activities of superoxide dismutase(SOD)and glutathione peroxidase(GPx)in antioxidants were significantly reduced(P<0.01),the expression levels of intestinal barrier proteins and short chain fatty acids(SCFAs)were significantly reduced(P<0.01).In comparison to MODEL group,the polyphenol intervention groups(RF and RP)showed significantly decreased levels of inflammatory factors and oxidative stress(P<0.05,P<0.01),The activity of antioxidant SOD and GPx in the RF group was significantly increased,and the levels of ZO-1,Claduin-1,and SCFAs in the gut of both the RF and RP groups were significantly reversed(P<0.01).Conclusion:Prickly pear polyphenol extract effectively alleviated metabolic disorders in high-fat diet-induced obese mice in vivo,resulting in reduced body weight gain,protection against cellular changes in adipose and hepatic tissues,alleviation of inflammation and oxidative stress,and enhanced intestinal barrier function.Notably,the RF group showed a superior effect to the RP group.

关 键 词：刺梨多酚提取物 肥胖小鼠 血脂 炎症因子 短链脂肪酸 

分 类 号：TS201.4[轻工技术与工程—食品科学]