麝香草酚控制核盘菌侵染不结球白菜效应研究  

Thymol controls the infection of Sclerotium sclerotiorum on Chinese cabbage

作　　者：杨立飞[1] 蔡金霞 石志琦[2] 宋立晓[2] 胡梁斌 陈健[2] YANG Lifei;CAI Jinxia;SHI Zhiqi;SONG Lixiao;HU Liangbin;CHEN Jian(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Science,Nanjing 210014,China;School of Food Science and Engineering,Shaanxi University of Science&Technology,Xi′an 710021,China)

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]江苏省农业科学院农产品质量安全与营养研究所,江苏南京210014 [3]陕西科技大学食品科学与工程学院,陕西西安710021

出　　处：《河南工业大学学报（自然科学版）》2024年第3期17-25,共9页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家重点研发计划项目(2022YFD2100601);现代农业产业体系项目(CARS-23-B16)。

摘　　要：核盘菌可侵染多种蔬菜,造成蔬菜采后品质下降,筛选高效安全的天然保鲜剂是控制核盘菌侵染的有效手段。通过离体和活体接种试验,研究了植物源天然化合物麝香草酚对核盘菌侵染青菜的控制效应。结果表明:20、40、60μg/mL麝香草酚处理后的菌丝鲜重分别比对照组显著下降了33.8%、53.7%、88.1%;菌丝干重分别比对照组下降了24.5%、53.7%、82.9%;麝香草酚处理导致核盘菌菌丝畸形、皱缩、细胞死亡,可能与菌丝细胞膜损伤有关,表现为相对电导率和丙二醛含量上升;麝香草酚还可诱导菌丝细胞甘油含量显著上升,发生渗透胁迫;50μg/mL麝香草酚处理青菜可显著抑制核盘菌在叶片上的侵染;在接种核盘菌的青菜叶片中,麝香草酚诱导特异型抗性基因BrMPK3和BrMPK4的表达量上调,并显著提升青菜品质(包括叶绿素、蛋白质、可溶性糖、维生素C含量提升)。麝香草酚可有效控制核盘菌对青菜的侵染,表现为抑菌效应和诱导寄主抗性效应。Sclerotium sclerotiorum can infect many vegetable species,which affects the quality of postharvest vegetables.Screening natural preservatives is an effective approach to control the infection of S.sclerotiorum.In this study,in vitro and in vivo inoculation experiments were conducted to investigate the effect of thymol(a plant-derived natural compound)on controlling the infection of S.sclerotiorum in Chinese cabbage.Thymol at different concentrations was added to the medium culturing S.sclerotiorum for in vitro experiment.Thymol inhibited mycelial growth in a dose-dependent manner.The fresh weight of S.sclerotiorum mycelia significantly decreased by 33.8%,53.7%,and 88.1%upon thymol treatment at 20,40,and 60μg/mL,respectively.The dry weight of S.sclerotiorum mycelia decreased by 24.5%,53.7%,and 82.9%upon thymol treatment at 20,40,and 60μg/mL,respectively.Thymol induced abnormality,break-down,and cell death of mycelia.The antifungal effect of thymol against S.sclerotiorum may result from cell membrane damage,supported by increases in mycelial relative conductivity and malonaldehyde content.Thymol also induced an increase in glycerol content in mycelia,indicating possible occurrence of osmotic stress.For in vivo experiment,S.sclerotiorum were inoculated on the leaves of Chinese cabbage followed by spraying thymol at 50μg/mL.Thymol significantly inhibited the infection of S.sclerotiorum on the leaves,with infection lesion significantly decreased by 30.1%as compared to water treatment.In the leaves inoculated with S.sclerotiorum,thymol induced significant increase in the expression of two genes BrMPK3 and BrMPK4 with specific role against S.sclerotiorum infection.Chinese cabbage quality was also enhanced by thymol,evidenced by the increase in the content of chlorophyll,protein,reduced sugar,and vitamin C.These results indicated that thymol effectively controlled the infection of S.sclerotiorum on the leaves of Chinese cabbage by inhibiting fungal growth and inducing host defense.This study provides scientific basis

关 键 词：核盘菌 青菜品质 麝香草酚 抑菌 植物抗性 

分 类 号：TS201.6[轻工技术与工程—食品科学]