锦灯笼果实总黄酮超声辅助提取工艺研究  

Study on Ultrasonic Assisted Extraction Process of Total Flavonoids from Jindeng Lantern Fruit

作　　者：徐丹鸿[1] 张桂娟[1] XU Dan-hong;ZHANG Gui-juan(Heilongjiang Vocational Institute of Ecological Engineering,Harbin 150025,China)

机构地区：[1]黑龙江生态工程职业学院生物技术系,黑龙江哈尔滨150025

出　　处：《黑龙江生态工程职业学院学报》2024年第4期47-51,共5页Journal of Heilongjiang Vocational Institute of Ecological Engineering

基　　金：中国(北方)现代林业职业教育集团科学研究资助课题项目“东北红铧头草功能活性成分及深加工产品研究”(LZJB2023KY006)。

摘　　要：锦灯笼果实中黄酮类化合物具有抗肿瘤、抗氧化、抗菌、抗炎等生物活性。以锦灯笼果实为原料,乙醇溶液为提取剂,锦灯笼果实总黄酮得率为评价指标,采用超声波辅助乙醇溶剂提取法,分别对超声功率、提取温度、提取时间、乙醇体积分数、料液比进行单因素影响试验。在单因素实验基础上,结合正交试验优化工艺参数。结果表明:影响锦灯笼果实总黄酮提取效果因素的主次顺序为提取时间>乙醇体积分数>超声功率>料液比。在提取温度50℃、提取时间30 min、乙醇体积分数70%和液料比为1∶30的条件下,锦灯笼果实总黄酮得率为3.32%。Flavonoids in the fruit of Jindeng lantern have biological activities such as anti-tumor,antioxidant,antibacterial,and anti-inflammatory.This study use Jindeng lantern fruit as the raw material,ethanol solution as the extraction agent,and the total flavonoid yield of Jindeng lantern fruit as the evaluation index.The ultrasound assisted ethanol solvent extraction method was used to conduct single factor impact experiments on ultrasound power,extraction temperature,extraction time,ethanol volume fraction,and solid-liquid ratio.On the basis of single factor experiments and combined with orthogonal experiments to optimize the process parameters,the results showed that the main and secondary order of factors affecting the extraction effect of total flavonoids from Jindeng lantern fruit was extraction time>ethanol volume fraction>ultrasound power>solid-liquid ratio.Under the conditions of extraction temperature of 50℃,extraction time of 30 min,ethanol volume fraction of 70%,and liquid to material ratio of 1∶30,the total flavonoid yield of Jindeng lantern fruit was 3.32%.

关 键 词：锦灯笼果实 总黄酮 超声辅助提取 正交试验 

分 类 号：TQ28[化学工程—有机化工]