忍冬叶总酚的提取及其抗氧化活性研究  

Extraction and Antioxidant Activity of Total Phenols From Leaves of Lonicera japonica Thunb

作　　者：张冬 贾莲 Zhang Dong;Jia Lian(North CMA Technology Co.,Ltd.,Shenyang Liaoning 110042,China;College of Chemistry and Life Science,Anshan Normal University,Anshan Liaoning 114007,China;Liaoning Key Laboratory of Development and Utilization for Natural Products Active Molecules,Anshan Liaoning 114007,China)

机构地区：[1]北方测盟测试技术(辽宁)有限公司,辽宁沈阳110042 [2]鞍山师范学院化学与生命科学学院,辽宁鞍山114007 [3]辽宁省天然产物活性分子开发及利用重点实验室,辽宁鞍山114007

出　　处：《山西化工》2024年第6期8-10,22,共4页Shanxi Chemical Industry

基　　金：辽宁省自然科学基金资助计划项目(2019-MS-001);辽宁省教育厅青年项目(扶持项目,jytqnfc202103)。

摘　　要：总酚是忍冬叶中主要的活性成分。本研究以忍冬叶为材料,采用乙醇为溶剂,结合超声波辅助法提取总酚,考察乙醇浓度、料液比和提取时间3个单因素对忍冬叶总酚得率的影响,利用正交实验对提取工艺进行优化,同时通过忍冬叶总酚对1,1-二苯基-2-三硝基苯肼(DPPH·)清除能力来评价其抗氧化活性。结果表明,最佳的工艺条件为乙醇体积分数70%、料液比1∶60、超声时间30 min时。在此条件下忍冬叶总酚提取率最高,为9.18 mg/g,忍冬叶提取物对DPPH·自由基清除率达到90.79%,具有较强的抗氧化能力。Total phenols are the main active ingredient in Lonicera japonica leaves.This study used L.japonica leaves as materials,ethanol as a solvent combined with ultrasonic assisted extraction of total phenols.The influence of three single factors of ethanol concentration,solid-liquid ratio,and extraction time on the extraction yield of total phenols from leaves of L.japonica was investigated.The technological factors affecting the extraction yield of total phenols from leaves of L.japonica was optimized by orthogonal method.The antioxidant activity was evaluated through the scavenging effect of total phenols from leaves of L.japonica on 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH·).Results showed that the optimal conditions of extracting total phenol from leaves of L.Japonica was ethanol concentration 70%,solid/liquid ratio 1:60 and extracting 30 min.Under these conditions the yield of total phenols was 9.18 mg/g.At this time,the DPPH·free radical scavenging rate of the total phenols from leaves of L.Japonica reached 90.79%,indicating strong antioxidant ability.

关 键 词：忍冬 总酚 超声提取 工艺优化 抗氧化活性 DPPH 

分 类 号：R284.2[医药卫生—中药学]