不同加工方式的油茶籽油对变应性接触性皮炎的抗炎效果  

Anti-inflammatory effect of oil-tea camellia seed oil extracted by different methods on allergic contact dermatitis

作　　者：陈宇[1] 杨纪元 邱之阳 张笑 邱昌扬 周凯 郝泽金 陈志敏 CHEN Yu;YANG Jiyuan;QIU Zhiyang;ZHANG Xiao;QIU Changyang;ZHOU Kai;HAO Zejin;CHEN Zhimin(Institute of Jiangxi Oil-tea Camellia/College of Pharmacy and Life Sciences/Jiujiang Key Laboratory of Food Processing and Safety,Jiujiang University,Jiujiang 332005,Jiangxi,China;Heyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Heyuan 517001,Guangdong,China;Jiangxi Sinomaster Oil Camellia Technology Co.,Ltd.,Jiujiang 332801,Jiangxi,China;Jiangxi Shengqi Agricultural Technology Co.,Ltd.,Jiujiang 332621,Jiangxi,China)

机构地区：[1]九江学院江西油茶研究中心/药学与生命科学学院/九江市食品加工与安全重点实验室,江西九江332005 [2]岭南现代农业科学与技术广东省实验室河源分中心,广东河源517001 [3]江西神州通油茶科技有限公司,江西九江332801 [4]江西晟启农业科技有限公司,江西九江332621

出　　处：《中国油脂》2024年第7期61-67,共7页China Oils and Fats

基　　金：岭南现代农业科学与技术广东省实验室河源分中心(DT20220005);江西省卫生健康委科技计划项目(SKJP220201588);江西省中医药管理局科技计划项目(2020A0373);江西省教育厅科学技术研究项目(GJJ211804);江西省林业局油茶专项项目(YCYJZX〔2023〕333)。

摘　　要：为了明确不同加工方式的油茶籽油对变应性接触性皮炎(ACD)的治疗效果,并探究油茶籽油中的主要抗炎物质,分别制备低温压榨油茶籽油、热榨油茶籽原油、热榨精炼油茶籽油、土榨油茶籽油和超临界CO_(2)油茶籽油,分析不同加工方式及添加不同活性物质的油茶籽油对ACD模型小鼠耳廓肿胀的影响,并结合ELISA法、HE染色和免疫组化探究其对小鼠炎症的抑制效果。结果表明:超临界CO_(2)油茶籽油对ACD小鼠耳廓肿胀的抑制率最高,与阳性对照组相比其小鼠血清中炎症因子TNF-α、IL-1β和IL-6含量降低率超过10%,炎症细胞浸润程度明显好转,耳廓肿胀组织中NF-κB阳性表达量与阴性对照组相比无显著差异;预防性给药的抗炎效果普遍低于造模后给药;倍数添加角鲨烯和生育酚的油茶籽油对ACD小鼠耳廓肿胀的抑制率显著提高,在50%左右,且角鲨烯、生育酚处理组小鼠耳廓组织中NF-κB阳性表达量与阴性对照组无显著差异。因此,外用超临界CO_(2)萃取的油茶籽油治疗小鼠ACD效果最佳,其抗炎效果并非源于单一活性成分,与角鲨烯和生育酚等活性成分均密切相关。In order to clarify the therapeutic effect of oil-tea camellia seed oil extracted by different methods on allergic contact dermatitis(ACD),and further explore the potential active substances of anti-inflammatory in oil-tea camellia seed oil,cold pressed oil-tea camellia seed oil,hot pressed crude oil-tea camellia seed oil,hot pressed-refined oil-tea camellia seed oil,native pressed oil-tea camellia seed oil,and supercritical CO_(2)extracted oil-tea camellia seed oil were prepared,the effects of different processing methods of oil-tea camellia seed oils and different active substances added with oil-tea camellia seed oils on auricular swelling in ACD model mice were analyzed,and their anti-inflammatory effect in mice were studied by combining ELISA method,HE staining,and immunohistochemistry.The results showed that the supercritical CO_(2)extracted oil-tea camellia seed oil showed the highest inhibition rate on auricular swelling of ACD mice,and the inflammatory factors,such as TNF-α,IL-1βand IL-6 were reduced more than 10%compared with positive control.The degree of inflammatory infiltration significantly improved,and there was no significant difference in NF-κB positive expression of auricular tissue between supercritical CO_(2)extracted oil-tea camellia seed oil treatment group and negative control.However,the anti-inflammatory effect of prophylactic administration was generally lower than that of post modeling administration.The oil-tea camellia seed oil that supplemented with multiple squalene and tocopherol increased the inhibition rate of auricular swelling about 50%.There was no significant difference in NF-κB positive expression of auricular tissue between the squalene and tocopherol group and the negative control.In general,supercritical CO_(2)extracted oil-tea camellia seed oil exhibits the best therapeutic effect on ACD mice,and the active anti-inflammatory ingredients are closely related to squalene and tocopherol.

关 键 词：油茶籽油 抗炎 变应性接触性皮炎 超临界CO_(2)萃取 

分 类 号：TS224[轻工技术与工程—粮食、油脂及植物蛋白工程] R758.22[轻工技术与工程—食品科学与工程]