副干酪乳杆菌VHProbi E12发酵藿香提取液的抗UVB性能研究  

The Performance of UVB Resistance of Lactobacillus paracasei VHProbi E12 Fermented Agastaggosa rugosa Extract

作　　者：李鑫萍 李凯玲 段治 LI Xin-ping;LI Kai-ling;DUAN Zhi(Nutrition&Health Technology Center,Qingdao Vland Biotech Inc.,Shandong Qingdao 266102,China)

机构地区：[1]青岛蔚蓝生物股份有限公司营养与健康技术中心,山东青岛266102

出　　处：《南昌航空大学学报（自然科学版）》2024年第2期69-75,共7页Journal of Nanchang Hangkong University(Natural Sciences)

摘　　要：中波紫外线(Ultraviolet B,UVB)照射会使细胞产生自由基,诱导细胞衰老和光老化。近年来,环境污染、臭氧层的破坏等问题导致UVB辐射增强,皮肤光老化问题日益严重。为筛选具有抗光老效果的藿香发酵菌株,建立人永生化角质形成细胞(Human immortalized keratinocytes,HaCaT)UVB损伤模型,并从细胞活力、抗光老、抗氧化以及抗炎效果方面评价菌株发酵液的作用效果。结果表明,副干酪乳杆菌VHProbi E12的发酵藿香提取液能够帮助HaCaT抵御UVB损伤,使细胞活力提高21.20%。在抗光老效果方面,与模型组相比,高浓度E12发酵液使基质金属蛋白酶-1含量减少24.68%,羟脯氨酸浓度提高43.79%。在抗氧化方面,E12发酵液处理可以逆转UVB照射引起的氧化损伤,显著提高细胞中SOD和GSH水平,与模型组相比,高浓度E12发酵液使SOD含量提高77.75%,GSH含量提高26.03%。此外,E12发酵液处理后,UVB照射引起的炎症也有所缓解,TNF-α和IL-6含量较未处理组降低。本研究提供了副干酪乳杆菌VHProbi E12发酵藿香提取液保护HaCaT抵御UVB损伤的证据,为藿香的进一步开发以及抗光老天然提取物在防晒产品方面的应用提供了理论依据。Ultraviolet B(UVB)irradiation can make cell generate free radicals,thereby leading to cellular senescence and photoaging.In recent years,due to environmental pollution and the destruction of the ozone layer,the intensity of UVB radiation has increased,exacerbating the problem of skin photoaging.To screen for fermented Agastache rugosa strains with anti-photoaging effects,a UVB damage model of human immortalized keratinocytes(HaCaT)was established.The efficacy of fermentation is evaluated based on cell viability,anti-photoaging,antioxidant and anti-inflammatory effects.The results indicate that Lactobacillus paracasei VHProbi E12 fermented Agastache rugosa extract helps HaCaT resist UVB damage and increases cell viability by 21.20%.In terms of anti-photoaging effect,high-dose E12 fermented Agastache rugosa extract reduces the matrix metalloproteinase-1content in HaCaT by 24.68%,and increases the hydroxyproline concentration by 43.79%compared with the model group.Regarding antioxidant capacity,E12 fermentation treatment can reverse the oxidative damage caused by UVB irradiation by elevating the levels of SOD acitivity and GSH content in cells.Compared with the model group,the high-dose E12 fermented Agastache rugosa extract increases the SOD by 77.75%and GSH content by 26.03%.Additionally,after E12 fermentation broth treatment,the inflammation caused by UVB irradiation is also alleviated,evidenced by reduced levels of TNF-αand IL-6 compared with the untreated group.This study provides evidence that a Agastache rugosa extract fermented by Lactobacillus paracasei VHProbi E12 protects HaCaT cells against UVB damage,and provides a theoretical basis for the further development of Agastache rugosa and the utilization of anti-photoaging natural extracts in sunscreen products.

关 键 词：藿香 副干酪乳杆菌 UVB损伤 抗光老 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]