A strategy for mechanically integrating robust hydrogeltissue hybrid to promote the anti-calcification and endothelialization of bioprosthetic heart valve  

作　　者：Haoshuang Wu Nuoya Chen Tiantian Zheng Li Li Mengyue Hu Yumei Qin Gaoyang Guo Li Yang Yunbing Wang 

机构地区：[1]National Engineering Research Center for Biomaterials,Sichuan University,Chengdu 610065,China [2]Institute of Clinical Pathology,West China Hospital of Sichuan University,Chengdu 610041,China [3]College of Polymer Science and Engineering,Sichuan University,Chengdu 610065,China

出　　处：《Regenerative Biomaterials》2024年第2期123-136,共14页再生生物材料（英文版）

基　　金：supported by National Key Research and Development Programs(2022YFB3807303 and 2022YFB3807305),National Natural Science Foundation of China(32101107)and CAMS InnovationFundforMedical Sciences(2021-12M-5-013)。

摘　　要：Bioprosthetic heart valve(BHV)replacement has been the predo-minant treatment for severe heart valve diseases over decades.Most clinically available BHVs are crosslinked by glutaraldehyde(GLUT),while the high toxicity of residual GLUT could initiate calcification,severe thrombosis,and delayed endothelializa-tion.Here,we construed a mechanically integrating robust hydrogel-tissue hybrid to improve the performance of BHVs.In particular,recombinant humanized coilagen type Ⅲ(rhCOLⅢ),which was precisely customized with anti-coagulant and pro-endothelialization bioactivity,was first incorporated into the polyvinyl alcohol(PVA)-based hydrogel via hydrogen bond interactions.Then,tannic acid was introduced to enhance the mechanicalperfo of PVA-based hvdrogel and interfacial bonding between the hydrogel layer and bio-derived tissue due to the strong affinity for a wide range of substrates.In vitro and in vivo experimental results confirmed that the GLUT-crosslinked BHVs modified by the robust PVA-based hydrogel embedded rhCOLII and TA possessed long-term anti-coagulant,accelerated endothelialization,mild inflammatory response and anti-calcification properties.Therefore,our mechanically integrating robust hydrogel-tissue hybrid strategy showed the potential to enhance the service function and prolong the service life of the BHVs after implantation.

关 键 词：bioprosthetic heart valves recombinant humanized collagen typeⅢ ENDOTHELIALIZATION ANTI-CALCIFICATION ANTI-COAGULATION 

分 类 号：TQ427.26[化学工程]