Genomics,transcriptomics,and peptidomics of the greater wax moth Galleria mellonella neuropeptides and their expression in response to lead stress  

作　　者：Li-Lin Luo Yang Lin Jun-Hong Linghu Wei Gong Yuan-Hong Luo Man Liu Dao-Chao Jin Guy Smagghe Tong-Xian Liu Shun-Hua Gui Tian-Ci Yi 

机构地区：[1]Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region,Institute of Entomology,Guizhou University,Guiyang,Guizhou,China [2]Guizhou Institute of Biology,Guizhou Academy of Sciences,Guiyang,China [3]Institute of Plant Health and Medicine,Guizhou University,Guiyang,China

出　　处：《Insect Science》2024年第3期773-791,共19页昆虫科学（英文版）

基　　金：supported in part by the National Natural Science Foundation of China(32202295);Guizhou Provincial Science and Technology Projects(Qian Ke He Ji Chu-ZK[2022]General 051);Scientific Research Foundation for Talent Introduced in Guizhou University(Gui Da Te Gang He Zi[2021]22);Guizhou Provincial Science and Technology Projects(Qian Ke He Support[2019]2292,Qian Ke He NY[2013]3040).

摘　　要：Neuropeptides are crucial in regulation of a rich variety of developmental,physiological,and behavioral functions throughout the life cycle of insects.Using an integrated approach of multiomics,we identified neuropeptide precursors in the greater wax moth Galleria mellonella,which is a harmful pest of honeybee hives with a worldwide distribution.Here,a total of 63 and 67 neuropeptide precursors were predicted and annotated in the G.mellonella genome and transcriptome,in which 40 neuropeptide precursors were confirmed in the G.mellonella peptidome.Interestingly,we identified 12 neuropeptide precursor genes present in G.mellonella but absent in honeybees,which may be potential novel pesticide target sites.Honeybee hives were contaminated with heavy metals such as lead,enabling its bioaccumulation in G.mellonella bodies through the food chain,we performed transcriptome sequencing to analyze the effects of Pb stress on the mRNA expression level of G.mellonella neuropeptide precursors.After treatment by Pb,the expression of neuropeptide F1 was found to be significantly downregulated,implying that this neuropeptide might be associated with responding to the heavy metal stress in G.mellonella.This study comprehensively identified neuropeptide precursors in G.mellonella,and discussed the effects of heavy metals on insect neuropeptides,with the example of G.mellonella.The results are valuable for future elucidation of how neuropeptides regulate physiological functions in G.mellonella and contribute to our understanding of the insect's environmental plasticity and identify potential new biomarkers to assess heavy metal toxicity in insects.

关 键 词：Galleria mellonella GENOMICS heavy metal stress NEUROPEPTIDE PEPTIDOMICS TRANSCRIPTOMICS 

分 类 号：Q96[生物学—昆虫学]