LAMA2在泛癌中的表达水平及其与预后、免疫微环境的相关性分析  

Expression level of LAMA2 in pan-cancer and its correlation with prognosis and immune microenvironment

作　　者：王一川 曹锐 陈正浩 田野[1] Wang Yichuan;Cao Rui;Chen Zhenghao;Tian Ye(Department of Urology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院泌尿外科,北京100050

出　　处：《国际外科学杂志》2024年第6期376-381,I0005-I0009,共11页International Journal of Surgery

基　　金：北京市教育委员会科学研究计划项目(KM202310025014)。

摘　　要：目的研究LAMA2基因在泛癌中,与生存时间、肿瘤微环境、免疫细胞浸润、免疫治疗反应性的相关性。方法从TCGA、IMvigor210数据库中下载LAMA2在泛癌中的表达矩阵及临床数据,利用R语言分析LAMA2表达水平与生存时间、肿瘤微环境、免疫细胞浸润、免疫治疗反应性的相关性,并对LAMA2进行基因富集分析,以探究相关通路。采用Wilcoxon检验比较LAMA2在肿瘤组织与正常组织中的表达差异,采用Kaplaner-Meier方法进行生存分析,采用单因素COX回归分析评价LAMA2在各个肿瘤中的风险比关系,采用Spearman相关性分析评价LAMA2的表达水平与肿瘤微环境免疫评分以及免疫细胞浸润的相关性,采用Preason相关性分析评价免疫治疗反应性与LAMA2表达水平的相关性。结果与正常组织相比,LAMA2在大多数癌种中的表达水平显著降低,此外,LAMA2的表达水平上调或下调对患者预后有提示作用。单因素COX回归分析结果表明,LAMA2在膀胱癌、肾乳头状细胞癌、肾上腺皮质癌等肿瘤中与预后显著相关。LAMA2的表达水平与肿瘤微环境评分、免疫评分与基质评分同样具有显著相关性。LAMA2表达水平高低与肿瘤组织免疫细胞(包括B细胞、CD8^(+)T细胞、调节性T细胞、肥大细胞等)浸润有相关性。基因富集分析表明,LAMA2在泛癌中大多参与离子通道、基因表达、骨骼肌运动的各个机制等生物学过程。LAMA2的表达还与肿瘤突变负荷、微卫星不稳定性及细胞程序死亡-配体1治疗反应性有关。结论LAMA2在肿瘤中对患者生存预后有预测价值,并与肿瘤微环境、免疫细胞浸润、免疫治疗反应性等有显著相关性,为潜在肿瘤标志物,为肿瘤的预后判断及治疗选择提供新的方向。Objective To investigate the relationship between the LAMA2 gene expression and the survival time of cancer patients,tumor microenvironment,tumor immune cell infiltration,and the immunotherapy responsiveness in pan-cancer.Methods Download the expression data matrix and the clinical data from TCGA and IMvigor210,furthermore analyze the correlation between the expression level of LAMA2 and survival time,tumor microenvironment,and immunotherapy responsiveness by R and perform the gene set enrichment analysis of LAMA2 in pan-cancer to explore the related pathways.The Wilcoxon test was used to compare the expression difference of LAMA2 between tumor tissues and normal tissues.Kaplan-Meier method was used for survival analysis,and univariate COX regression analysis was used to evaluate the risk ratio relationship of LAMA2 in various tumors.Spearman correlation analysis was used to evaluate the correlation between the expression level of LAMA2 and tumor microenvironment immune score,as well as immune cell infiltration.Pearson correlation analysis was used to evaluate the correlation between the immunotherapy responsiveness and the expression level of LAMA2.Results Compared with normal tissues,the expression level of LAMA2 was significantly decreased in most cancer types.In addition,the up-regulation or down-regulationand of LAMA2 expression may indicate the prognosis of patients.Univariate COX regression analysis showed that LAMA2 was significantly associated with prognosis in bladder cancer,renal papillary cell carcinoma,adrenocortical carcinoma,and so on.LAMA2 expression level was also significantly correlated with tumor microenvironment score,immune score and stromal score.Furthermore,the expression level of LAMA2 was correlated with the infiltration of tumor immune cells,including B cell,CD8^(+)T cell,regulatory T cell,mast cell,and so on.The results of gene set enrichment analysis show that the LAMA2 was mostly involved in the biological process of ion channels,gene expression,and skeletal muscle movement.LAMA2 exp

关 键 词：计算生物学 免疫 治疗学 LAMA2 肿瘤微环境 

分 类 号：R730.5[医药卫生—肿瘤]