Melatonin and dopamine alleviate waterlogging stress in apples by recruiting beneficial endophytes to enhance physiological resilience  

作　　者：Yang Cao Peihua Du Yuwei Shang Jiahao Ji Leiqing Tan Xue Zhang Jizhong Xu Bowen Liang 

机构地区：[1]College of Horticulture,Hebei Agricultural University,Baoding 071001,China

出　　处：《Journal of Integrative Agriculture》2024年第7期2270-2291,共22页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31901964);the Natural Science Foundation of Hebei,China(C2021204158);the Science and Technology Project of Hebei Education Department,China(BJK2022012);the Introduced Talents Project of Hebei Agricultural University,China(YJ201904);the earmarked fund for Hebei Apple Innovation Team of Modern Agroindustry Technology Research System,China(HBCT2024150205);the earmarked fund for the China Agricultural Research System,China(CARS-27).

摘　　要：Melatonin and dopamine can potentially prevent waterlogging stress in apples.The current study investigated the mechanism by which melatonin and dopamine alleviate apple waterlogging stress.This study demonstrated that melatonin and dopamine alleviated waterlogging by removing reactive oxygen species(ROS),and that the nitric oxide(NO)content and nitrate reductase(NR)activity were significantly correlated.Melatonin and dopamine were also found to recruit different candidate beneficial endophytes(melatonin:Novosphingobium,Propionivibrio,and Cellvibrio;dopamine:Hydrogenophaga,Simplicispira,Methyloversatilis,Candidatus_Kaiserbacteria,and Humicola),and these endophytes were significantly and positively correlated with plant growth.Network analyses showed that melatonin and dopamine significantly affected the endophytic bacterial and fungal communities under waterlogging stress.The metabolomic results showed that melatonin and dopamine led to waterlogging resistance by upregulating the abundance of beneficial substances such as amino acids,flavonoids,coumarins,and organic acids.In addition,melatonin and dopamine regulated the physicochemical properties of the soil,which altered the endophyte community and affected plant growth.The co-occurrence network demonstrated close and complex relationships among endophytes,metabolites,soil,and the plants.Our results demonstrate that melatonin and dopamine alleviate waterlogging stress in apples by recruiting beneficial endophytes to enhance physiological resilience.This study provides new insights into how melatonin and dopamine alleviate stress and a theoretical basis for synergistic beneficial microbial resistance to waterlogging stress.

关 键 词：DOPAMINE ENDOPHYTES Malus hupehensis MELATONIN metabolomics WATERLOGGING 

分 类 号：S661.1[农业科学—果树学] S422[农业科学—园艺学]