一起非O1/O139群霍乱弧菌引起的霍乱病原学特征分析  

Analysis of pathogenic characteristics of cholera caused by non-O1/O139 Vibrio cholera

作　　者：吴春敏 胡凤清[1] 刘承毅 罗燕 WU Chun-min;HU Feng-qing;LIU Cheng-yi;LUO Yan(Nanping Municipal Center for Disease Control and Prevention,Nanping,Fujian 353000,China)

机构地区：[1]福建省南平市疾病预防控制中心,福建南平353000

出　　处：《疾病预防控制通报》2024年第3期6-9,共4页Bulletin of Disease Control & Prevention(China)

摘　　要：目的了解非O1/O139群霍乱弧菌基因序列和耐药等病原特征,为霍乱基因组学等提供资料,为处置类似事件提供参考。方法对患者开展流行病学调查并采集粪便进行分离培养,用生化试验和基质辅助激光解吸电离飞行时间质谱(布鲁克MALDI Biotype sirius one)鉴定,并用玻片凝集法进行血清群/型鉴定;用微量肉汤稀释法进行药敏试验;利用Illumina Hiseq^(TM)测序平台进行细菌16S rRNA基因扩增及全基因组测序;对菌株开展毒力基因和耐药基因检测与分析。结果该患者曾饮用生水,同住家人、邻居(曾到过患者家就餐)以及同单元5层10户37人未见发病。患者粪便标本检出非O1/O139群霍乱弧菌,该菌携带致病基因且对多粘菌素E耐药。使用PubMLST分型数据库鉴定该菌株为ST841型,且为新型。通过比对获得最近缘的序列为霍乱弧菌参考菌株ERR1024532/1995/Latin America/non O1,与最近缘基因组序列的核基因数目差为328。结论本病例感染病原为非O1/O139群霍乱弧菌;应加强霍乱弧菌的食品安全风险监测以及食源性疾病疫情监测与报告,预防食源性疾病暴发。Objective To understand the gene sequence and drug resistance characteristics of non-O1/O139 group V.cholera,and provide information for cholera genomics,so as to provide reference for handling emergency.Methods Epidemiological investigation was carried out among patients and the feces samples were collected for isolation and culture.Biochemical tests and matrix-assisted laser desorption ionization time-of-flight mass spectrometry(Bruker MALDI Biotype sirius one)were used for identification,and slide agglutination was used for serogroup/type identification.The drug susceptibility test was carried out with microbroth dilution method.The Illumina Hiseq^(TM) sequencing platform was used for bacterial 16S rRNA gene amplification and whole genome sequencing.Virulence gene and drug resistance gene were detected and analyzed.Results The patient drank un-boiled water.There was no patient found in family members,neighbors(who dined at the patient's home),and 37 people from 10 households on the 5th floor of the same unit.In the patient's feces samples,non-O1/O139 V.cholera was detected with pathogenic gene and resistance to polymyxin E.The strain was identified as ST841,a new type of strain by PubMLST typing database.The sequence of the closest edge obtained by comparison was V.cholerae reference strain ERR1024532/1995/Latin America/non O1.The difference between the number of nuclear genes and the nearest genome sequences was 328.Conclusions The pathogen of this patient is non-O1/O139 V.cholera.The monitoring of food safety of V.cholera and foodborne disease surveillance and report should be strengthened for outbreak prevention of foodborne diseases.

关 键 词：非O1/O139群霍乱弧菌 药敏试验 毒力基因 全基因组测序 

分 类 号：R155.31[医药卫生—营养与食品卫生学]