B.t.微胶囊的喷雾干燥制备工艺优化及性能评价  

Process optimization and performance evaluation of B.t.microcapsule prepared by spray drying

作　　者：陈蔚燕[1] 高明惠 陈泳汀 辛露露 郑桂玲 徐晓庆 曲田丽[1] 张保华[1] CHEN Weiyan;GAO Minghui;CHEN Yongting;XIN Lulu;ZHENG Guiling;XU Xiaoqing;QU Tianli;ZHANG Baohua(Qingdao Agricultural University,College of Chemistry and Pharmaceutical Sciences,,Qingdao 266109;Qingdao Agricultural University,College of Plant Health and Medicine,Qingdao 266109,Shandong,China;Rushan Hanwei Biotechnology Co.,Ltd.,Weihai 264500,Shandong,China)

机构地区：[1]青岛农业大学化学与药学院,山东青岛266109 [2]青岛农业大学植物医学学院,山东青岛266109 [3]乳山韩威生物科技有限公司,山东威海264500

出　　处：《农药》2024年第7期494-499,531,共7页Agrochemicals

基　　金：国家重点研发项目(2022YFD2300100);山东省科技型中小企业创新能力提升工程项目(2022TSGC1311);青岛市科技惠民示范专项(24-1-8-xdny-10-nsh);山东省自然科学基金资助项目(ZR2021MC108,ZR2021MC072)。

摘　　要：[目的]为保证苏云金芽孢杆菌Bacillus thuringiensis(B.t.)制剂的储藏稳定性和抗逆性,从而充分发挥其杀虫作用、延长田间存活能力,制备了B.t.微胶囊。[方法]以B.t新菌株(B-Y7-1)为研究对象,采用喷雾干燥技术制备B.t.微胶囊,通过单因素筛选及正交优化试验确定了喷雾干燥法的最佳制备工艺,并对其性能和活性进行了表征和评价。[结果]B.t.微胶囊喷雾干燥法的最佳工艺为:3%海藻酸钠为壁材,进口温度180℃、物料流速14 mL/min,微胶囊产率为76.45%±1.35%,活菌率为93.11%±2.03%;54℃下热储14 d后,喷雾干燥法制备的微胶囊囊内的芽孢存活率为71.23%,远高于B.t.菌粉的存活率8.08%;B.t.微胶囊在水中的释放符合一级动力学模型,在土壤中释放符合Ritger-Peppas模型,属于Fick扩散。生物活性测定表明,B.t.微胶囊对蛴螬的持效杀虫效果高于未包埋的B.t.菌液。[结论]实验结果说明B.t.微胶囊化可以改善其稳定性,并具有缓释特性。[Aims]To ensure the storage stability and stress resistance of Bacillus thuringiensis(B.t.)formulations,fully utilize their insecticidal effect,and extend their field survival capacity,B.t.microcapsules were prepared.[Methods]B.t.(B-Y7-1)microcapsules were prepared by using spray drying technology.Through single factor screening and orthogonal optimization tests,the best preparation process of spray drying method was determined,and its performance and activity were characterized and evaluated.[Results]The best process of spray drying was:3%of sodium alginate as wall material,inlet temperature of 180°C,material flow rate of 14 mL/min,with the microcapsule yield of 76.45%±1.35%and the viable rate of 93.11%±2.03%.After 14 d of thermal storage at 54°C,the survival rate of spores in the microcapsules prepared by spray drying method was 71.23%,which was much higher than that of B.t.powder(8.08%).The release of B.t.microcapsules in water followed a first-order kinetic model,while in soil it followed the Ritger-Peppas model and belongs to Fick diffusion.The biological activity test showed that the sustained insecticidal effect of B.t.microcapsules on white grubs was higher than that of non-embedded B.t.bacterial solution.[Conclusions]The results indicated that B.t.microcapsule can improve its stability and has sustained-release properties.

关 键 词：苏云金芽孢杆菌 微胶囊 喷雾干燥 性能 

分 类 号：TQ450.6[化学工程—农药化工]