大果木姜子油的提取工艺优化及其对心肌缺血改善作用的研究  

Optimization of the extraction process of Cinnamomum migao oil and its ameliorative effects on myocardial ischemia

作　　者：孙巡巡 徐剑 刘杰 郭江涛 朱小婷 SUN Xun-Xun;XU Jian;LIU Jie;GUO Jiang-Tao;ZHU Xiao-Ting(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;National Engineering Research Center of Miao Medicine,Guiyang 550025,China;Guizhou Engineering Research Center of Traditional Chinese Medicine Processing and Preparation,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵阳550025 [2]国家苗药工程技术研究中心,贵阳550025 [3]贵州中药炮制与制剂工程技术研究中心,贵阳550025

出　　处：《食品安全质量检测学报》2024年第11期262-273,共12页Journal of Food Safety and Quality

基　　金：贵州省基础研究项目(自然科学)(黔科合基础-ZK[2022]一般463);贵科合学术新苗项目([2023]-12号);国家自然科学基金委员会-贵州省人民政府联合基金项目(U1812403-2);贵州省高层次创新型人才项目(黔科合平台人才-GCC[2023]037)。

摘　　要：目的采用响应面法优化大果木姜子油的提取工艺,并探讨不同来源大果木姜子油对心肌缺血的改善作用。方法采用单因素实验法考察提取溶剂、液料比、物料粒径、提取温度和提取时间5个单因素对大果木姜子油得率的影响,并在此基础上运用响应面法就液料比、物料粒径和提取温度3个因素进行大果木姜子油提取工艺的优化。建立大鼠心肌缺血模型,采用免疫印迹法检测大鼠心肌组织中兔抗人单克隆抗体(Bcl-2 assaciated xprotein,Bax)及B淋巴细胞瘤-2基因(B-cell lymphoma-2,Bcl-2)蛋白的表达、采用聚合酶链式反应检测心肌组织中碱性成纤维生长因子(fibroblast growth factor 2,FGF-2)的表达、采用免疫组化法检测心肌组织中纤溶酶原激活物抑制剂-1(plasminogen activator inhibitor-1,PAI-1)及组织型纤溶酶原激活剂(tissue-type plasminogenactivator,TPA)的表达,并采用苏木精-伊红染色法观察心肌组织病理变化。结果经响应面法分析大果木姜子油的最佳提取工艺为:液料比7:1(mL:g),粒径过80目,提取温度90℃,得率为51.23%。药理实验表明:与空白组相比,模型组中Bcl-2、FGF-2和TPA的水平下降,而Bax和PAI-1水平上升,经大果木姜子油治疗后Bax、PAI-1的水平下降,而FGF-2、TPA和Bcl-2水平上升。结论响应面法优化后的大果木姜子油的提取工艺条件稳定、可行,且大果木姜子油具有改善心肌缺血的作用。本研究为提取和应用大果木姜子油提供了坚实的理论基础。Objective To optimize the extraction process of Cinnamomum migao oil using response surface methodology and explore the improvement effects of different sources of Cinnamomum migao oil on myocardial ischemia.Methods A single factor experimental method was used to investigate the effects of 5 single factors,namely extraction solvent,liquid-solid ratio,material particle size,extraction temperature,and extraction time,on the yield of Cinnamomum migao oil.Based on this,response surface methodology was used to optimize the extraction process of Cinnamomum migao oil using 3 factors:Liquid-solid ratio,material particle size,and extraction temperature.Establishing a rat myocardial ischemia model,western blot(WB)was used to detect the expression of Bcl-2 assaciated xprotein(Bax)and B-cell lymphoma-2(Bcl-2)protein in rat myocardial tissue,polymerase chain reaction(PCR)was used to detect the expression of fibroblast growth factor 2(FGF-2)in myocardial tissue,and immunohistochemistry(IHC)was used to detect the expression of plasminogen activator inhibitor-1(PAI-1)and tissue-type plasminogenactivator(TPA)in myocardial tissue,hematoxylin eosin staining(HE)was used to observe the pathological changes of myocardial tissue.Results The optimal extraction process for Cinnamomum migao oil was as follows:liquid-solid ratio 7:1(mL/g),particle size over 80 mesh,extraction temperature 90℃,and yield of 51.23%.Pharmacological experiments had shown that compared with the blank group,the levels of Bcl-2,FGF-2,and TPA in the model group decreased,while the levels of Bax and PAI-1 increased.After treatment with Cinnamomum migao oil,the levels of Bax and PAI-1 decreased,while the levels of FGF-2,TPA,and Bcl-2 increased.Conclusion The extraction process conditions of Cinnamomum migao oil optimized by response surface methodology are stable and feasible,and Cinnamomum migao oil has the effect of improving myocardial ischemia.This study provides a solid theoretical basis for the extraction and application of Cinnamomum migao oil.

关 键 词：大果木姜子油 提取工艺 响应面优化 心肌缺血 

分 类 号：TS201.4[轻工技术与工程—食品科学]