乳酸对小鼠未成熟树突状细胞影响的基因芯片分析  

Gene chip analysis of the effects of lactate acid on murine immature dendritic cells

作　　者：赵茂 黄瑾 刘新宇 邱炜 ZHAO Mao;HUANG Jin;LIU Xinyu;QIU Wei(School of Biology and Engineering(College of Health and Medicine Modern Industry),Guizhou Medical University,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州医科大学生物与工程学院(健康医药现代产业学院),贵州贵阳550025

出　　处：《贵州师范大学学报（自然科学版）》2024年第4期108-116,共9页Journal of Guizhou Normal University：Natural Sciences

基　　金：国家自然科学基金(31960206)。

摘　　要：研究乳酸对小鼠未成熟树突状细胞(imDCs)基因组表达水平的影响,为深入研究病理酸性微环境对imDCs免疫功能影响的分子机制提供前期数据。从C57BL/6J小鼠骨髓中分离单核细胞,并诱导其分化为imDCs。20 mmol/L浓度乳酸处理imDCs 24 h,利用BGISEQ平台进行基因芯片分析,筛选出差异表达基因。结果显示,20 mmol/L浓度乳酸处理使imDCs中表达发生变化的基因有915个。通过基因本体论(GO)分析发现差异表达基因参与细胞骨架、凋亡过程及免疫反应等;京都基因与基因组百科全书(KEGG)分析发现差异表达基因涉及的信号通路包括MAPK信号通路、PI3K-AKT信号通路及TOLL样受体信号通路等。利用Cytoscape软件构建差异表达蛋白之间的互作网络图,并根据节点数筛选关键差异表达蛋白。筛选出分化抗原簇38(CD38)、3-羟基-3-甲基戊二酰辅酶A还原酶(HMGCR)等上调关键差异表达蛋白和信号转导和趋化因子受体7(CCR7)、转录激活因子1(STAT1)等下调关键差异表达蛋白。The study aims to investigate the effect of lactate acid on the genomic expression levels of immature dendritic cells(imDCs)in mice,providing preliminary data for further understanding the molecular mechanisms underlying the impact of pathological acidic microenvironments on the immune function of imDCs.mononuclear cells were isolated from C57BL/6J mouse bone marrow to induce their differentiation into imDCs.By treating imDCs with 20 mmol/L concentration of lactic acid for 24 h,followed by gene chip analysis using the BGISEQ platform,differentially expressed genes were identified.The results showed that treatment with a concentration of 20 mmol/L lactic acid resulted in changes in the expression of 915 genes in imDCs.Through Gene ontology(GO)analysis,it was found that the differentially expressed genes were involved in processes such as cell cytoskeleton,apoptosis,and immune response.Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis revealed that the differentially expressed genes were related to signaling pathways including MAPK signaling pathway,PI3K-AKT signaling pathway,and Toll-like receptor signaling pathway.A network diagram of interactions among differentially expressed proteins was constructed using by using Cytoscape software,and key differentially expressed proteins were filtered based on node connectivity.Up-regulated key differentially expressed proteins such as cluster of differentiation 38(CD38)and 3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMGCR)were identified,along with down-regulated key differentially expressed proteins such as chemokine c-c-motif receptor 7(CCR7)and signal transducer and activator of transcription 1(STAT1).

关 键 词：乳酸 未成熟树突状细胞 基因芯片 GO分析 KEGG分析 

分 类 号：R363[医药卫生—病理学]