Light-Mediated Spatiotemporally Dynamic Assembly of DNA Nanostructures in Living Cells Regulates Autophagy  

作　　者：Rui Zhang Pin Hu Yuwei Xu Ziqing Wang Dayong Yang Chi Yao 

机构地区：[1]Frontiers Science Center for Synthetic Biology,Key Laboratory of Systems Bioengineering(MOE),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350 [2]Department of Chemistry,State Key Laboratory of Molecular Engineering of Polymers,Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials,College of Chemistry and Materials,Fudan University,Shanghai 200438

出　　处：《CCS Chemistry》2024年第6期1557-1570,共14页中国化学会会刊（英文）

基　　金：supported by the National Natural Science Foundation of China(grant nos.22225505,22322407,and 22174097);D.Y.thanks Fudan University Ruiqing Education Funding.

摘　　要：The assembly of exogenous artificial architectures inside cells can regulate a series of biological events,which heavily relies on the development of spatiotemporally controlled molecular assembly systems.We herein report a designer deoxyribonucleic acid(DNA)nanostructure that enables light-mediated spatiotemporally dynamic assembly in living cells and consequently achieves efficient regulation of cell autophagy.The DNA nanostructure was constructed from i-motif moiety-containing branched DNA,photocleavable bond-containing linker,and tumor cell-targeting aptamer.After cellular uptake mediated by aptamers,under the spatiotemporal control of both UV light and late endosomal/lysosomal acidic environments,disassembly/reassembly of DNA nanostructure occurred via two rationally designed routes,generating microsized DNA assembly.As a result,autophagy was significantly enhanced with the increase of DNA assembly size.The enhanced autophagy showed an impact on related biological effects.Our system is expected to be a powerful tool for the regulation of intracellular events and cellular behaviors.

关 键 词：DNA nanotechnology DNA hydrogel SELF-ASSEMBLY AUTOPHAGY I-MOTIF 

分 类 号：TB383[一般工业技术—材料科学与工程]