优化法结合制备型高效液相色谱分离纯化虾青素  

Separation and Purification of Astaxanthin by Optimization Methodology Combined with Preparative High Performance Liquid Chromatography

作　　者：高红 张炜 靳明坤 GAO Hong;ZHANG Wei;JIN Mingkun(School of Chemistry and Chemical Engineering,Qinghai Normal University,Xining 810008,Qinghai,China;Xining Natural Resources Comprehensive Survey Center of China Geological Survey,Xining 810000,Qinghai,China)

机构地区：[1]青海师范大学化学化工学院,青海西宁810008 [2]中国地质调查局西宁自然资源综合调查中心,青海西宁810000

出　　处：《食品研究与开发》2024年第14期119-126,共8页Food Research and Development

基　　金：战略性矿产勘查岩矿测试技术支撑(西宁中心)项目(DD20243421)。

摘　　要：以雨生红球藻中的虾青素为研究对象,研究其虾青素的皂化工艺条件,通过单因素试验和响应面优化法进行NaOH-甲醇溶液皂化工艺优化,并通过制备型高效液相色谱纯化。最终得出的最佳工艺条件为皂化时间12 h、皂化温度32℃、NaOH-甲醇溶液浓度2.5%,在此条件下测得虾青素含量为(19.423±0.186)μg/mL。进一步通过制备型高效液相色谱对皂化得到的虾青素纯化处理,经液相色谱测定虾青素的纯度达94%以上,利用红外光谱和核磁共振对其纯化产物进行定性分析,通过图谱分析发现制备得到的虾青素与标准品虾青素图谱基本一致。The study focused on astaxanthin extracted from Haematococcus pluvialis and optimized its saponification process.Through single-factor experiments and response surface optimization,the saponification process using NaOH-methanol solution was optimized,followed by purification using preparative high performance liquid chromatography(HPLC).The optimal conditions obtained were saponification time of 12 h,saponification temperature of 32℃,and NaOH-methanol solution concentration of 2.5%.Under these conditions,the astaxanthin content was measured to be(19.423±0.186)μg/mL.Further purification of the saponified astaxanthin was achieved through preparative HPLC,resulting in a purity of over 94% as determined by liquid chromatography.Qualitative analysis of the purified product was conducted using infrared spectroscopy and nuclear magnetic resonance,and spectral analysis revealed a close similarity between the prepared astaxanthin and the standard astaxanthin.

关 键 词：雨生红球藻 虾青素 皂化 制备型高效液相色谱 纯化 

分 类 号：TS201.2[轻工技术与工程—食品科学] O652.63[轻工技术与工程—食品科学与工程]