SV2B defines a subpopulation of synaptic vesicles  

作　　者：Isabelle Paulussen Hannes Beckert Timothy FMusial Lena J.Gschossmann Julia Wolf Mathieu Schmitt Jérôme Clasadonte Georges Mairet-Coello Christian Wolff Susanne Schoch Dirk Dietrich 

机构地区：[1]Synaptic Neuroscience Team,Department of Neurosurgery,University Hospital Bonn,Bonn 53127,Germany [2]Synaptic Neuroscience Team,Department of Neuropathology,University Hospital Bonn,Bonn 53127,Germany [3]Microscopy Core Facility,Medical Faculty,University of Bonn,Bonn 53127,Germany [4]UCB Pharma,Braine l’Alleud 1420,Belgium

出　　处：《Journal of Molecular Cell Biology》2023年第9期1-13,共13页分子细胞生物学报（英文版）

基　　金：supported by grants from Deutsche Forschungsgemeinschaft(DFG)(SFB1089,SCHO 820/4-1,SCHO 820/6-1,SCHO 820/7-1,SCHO 820/5-2,and SPP1757 to S.S.,SFB1089,SPP1757,INST117215,DI853/3-5&7,and INST 217/785-1 to D.D.),the BONFOR program of the University of Bonn Medical Center(S.S.and D.D.),and UCB Pharma.

摘　　要：Synaptic vesicles can undergo several modes of exocytosis,endocytosis,and trafficking within individual synapses,and their fates may be linked to different vesicular protein compositions.Here,we mapped the intrasynaptic distribution of the synaptic vesicle proteins SV2B and SV2A in glutamatergic synapses of the hippocampus using three-dimensional electron microscopy.SV2B was almost completely absent from docked vesicles and a distinct cluster of vesicles found near the active zone.In contrast,SV2A was found in all domains of the synapse and was slightly enriched near the active zone.SV2B and SV2A were found on the membrane in the peri-active zone,suggesting the recycling from both clusters of vesicles.SV2B knockout mice displayed an increased seizure induction threshold only in a model employing high-frequency stimulation.Our data show that glutamatergic synapses generate molecularly distinct populations of synaptic vesicles and are able to maintain them at steep spatial gradients.The almost complete absence of SV2B from vesicles at the active zone of wildtype mice may explain why SV2A has been found more important for vesicle release.

关 键 词：3D electron microscopy synaptic vesicles synaptic vesicle proteins ENDOCYTOSIS TRAFFICKING spatial organization 

分 类 号：O62[理学—有机化学]