基于多克隆抗体的糠氨酸ic-ELISA检测方法的建立  

Development of ic-ELISA for Furosine Detection Based on Polyclonal Antibodies

作　　者：杨敏仪 罗雯倩 贾荣业 刘宇轩 王荣智 YANG Minyi;LUO Wenqian;JIA Rongye;LIU Yuxuan;WANG Rongzhi(College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Key Laboratory of Biopesticide and Chemical Biology,Ministry of Education,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建农林大学生物农药与化学生物学教育部重点实验室,福建福州350002 [3]福建农林大学福建省病原真菌与真菌毒素重点实验室,福建福州350002

出　　处：《食品与生物技术学报》2024年第5期138-145,共8页Journal of Food Science and Biotechnology

基　　金：福建省科技厅高校产学合作项目(2021N5009)。

摘　　要：采用甲醛法制备了糠氨酸完全抗原,通过交叉验证确保了多克隆抗体的特异性,从而建立了基于该抗体的糠氨酸间接竞争酶联免疫吸附测定(indirect competitive enzyme-linked immunosorbent assay,ic-ELISA)检测方法。结果显示,建立的ic-ELISA检测方法有效检测范围为12.20~2143.42 ng/mL,最低检测限为2.69 ng/mL。该方法操作简便、迅速,成本低廉,同时具备高灵敏度和优异特异性,为高效检测食品中糠氨酸质量浓度提供了新的选择,具有一定实际应用价值。A complete furosine complete antigen was prepared using the formaldehyde method,and cross-verification was employed to ensure the specificity of the polyclonal antibodies.An indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)was successfully established based on the antibodies.The results demonstrated that the established ic-ELISA exhibited a detection range of 12.20 to 2143.42 ng/mL,with a limit of detection(LOD)at 2.69 ng/mL.This method is simple,rapid,and cost-effective,offering high sensitivity and excellent specificity.It provides a novel option for the efficient detection of furosine in food,demonstrating its potential for practical application.

关 键 词：糠氨酸 动物免疫 多克隆抗体 ic-ELISA 

分 类 号：TS207[轻工技术与工程—食品科学]