羊栖菜多糖及辅助超声降解对高脂斑马鱼的降血脂作用  

Hypolipidemic Effect of Sargassum fusiforme Polysaccharide and Ultrasonic-Assisted Degradation on Hyperlipidemic Zebrafish

作　　者：李文青 祝孙婷 李圣洁 李梦瑶 章航嘉 顾青[1] 宋达峰[1] LI Wenqing;ZHU Sunting;LI Shengjie;LI Mengyao;ZHANG Hangjia;GU Qing;SONG Dafeng(School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China)

机构地区：[1]浙江工商大学食品与生物工程学院,浙江杭州310018

出　　处：《食品与生物技术学报》2024年第5期156-163,共8页Journal of Food Science and Biotechnology

基　　金：温州市重大科技创新攻关项目(ZN2023002)。

摘　　要：研究了羊栖菜多糖(Sargassum fusiforme polysaccharide,SFPS)及辅助超声降解对模式生物斑马鱼幼鱼和成鱼的降血脂作用。将斑马鱼幼鱼分为对照组、高脂组、200 mg/L超声降解羊栖菜多糖组(200-SFPSUD)、600 mg/L SFPSUD组(600-SFPSUD)和600 mg/L SFPS组(600-SFPS),实验周期为24 h。斑马鱼成鱼分为对照组、高脂组、30 mg羊栖菜粉组(SFP)、20 mg/L SFPSUD组(20-SFPSUD)、50 mg/L SFPSUD组(50-SFPSUD)和50 mg/L SFPS组(50-SFPS),实验周期为28 d。在斑马鱼幼鱼实验中,600-SFPS组斑马鱼较高脂组血管和腹部脂肪积累较少,600-SFPSUD组斑马鱼体内的脂肪积累更少。斑马鱼成鱼实验中,较高脂组,50-SFPS组斑马鱼肥满度变化率降低25.53%,肝脂酶(HL)、总脂酶(TL)活性分别提高153.00%、123.48%;50-SFPSUD组斑马鱼肥满度变化率降低27.18%,HL、TL活性分别提高168.20%、137.73%。通过苏木精-伊红染色(HE)观察到50-SFPS组和50-SFPSUD组斑马鱼肝脏内脂肪堆积均得到有效抑制,其中SFPSUD的干预效果更为显著。结果表明,SFPS具有降血脂功能,超声降解处理能够提高其降血脂能力,其机制可能是提高了斑马鱼HL和TL活性,从而加速体内脂质代谢速率。该研究可为SFPS、超声降解功能性食品的进一步开发提供理论参考,为人类高脂血症和养殖鱼类肥胖等问题提供新的解决思路。This study investigated the hypolipidemic effects of Sargassum fusiforme polysaccharide(SFPS)and ultrasonic-assisted degradation on both juvenile and adult zebrafish,serving as model organisms.Juvenile zebrafish were divided into control group,high-fat group,200 mg/L ultrasonic degradation of Sargassum fusiforme polysaccharide(SFPSUD)group(200-SFPSUD),600 mg/L SFPSUD group(600-SFPSUD),and 600 mg/L SFPS group(600-SFPS)with an experimental period of 24 h.Adult zebrafish were divided into control group,high-fat group,30 mg Sargassum fusiforme powder group(SFP),20 mg/L SFPSUD group(20-SFPSUD),50 mg/L SFPSUD group(50-SFPSUD),and 50 mg/L SFPS group(50-SFPS)with an experimental period of 28 d.In the experiment with juvenile zebrafish,the zebrafish in 600-SFPS group exhibited less accumulation of fat in the blood vessels and abdomen than those in the high-fat group,while the zebrafish in 600-SFPSUD group showed the least fat accumulation.In the experiment with adult zebrafish,compared to the high-fat group,the 50-SFPS group showed a decrease in the body fat change rate by 25.53%,while the activities of hepatic lipase(HL)and total lipase(TL)increased by 153.00%and 123.48%,respectively.The 50-SFPSUD group exhibited a decrease in the body fat change rate by 27.18%,with HL and TL activities increased by 168.20%and 137.73%,respectively.Hematoxylin-eosin staining(HE)demonstrated effective inhibition of fat accumulation in the liver of zebrafish in both the 50-SFPS and 50-SFPSUD groups,with the intervention of SFPSUD being more significant.The above results show that Sargassum fusiforme polysaccharide has the function of reducing blood lipid.Ultrasonic degradation can improve the hypolipidemic capability of Sargassum fusiforme polysaccharide.The mechanism may be to improve the activities of TL and HL of zebrafish,so as to accelerate the metabolic rate of lipid in vivo.This study can provide theoretical reference for the further development of SFPS and ultrasonic-degraded functional foods,and provide new solutions to issues such

关 键 词：羊栖菜多糖 超声降解 斑马鱼 降血脂 

分 类 号：TS201.4[轻工技术与工程—食品科学]