Exosomes from umbilical cord mesenchymal stromal cells promote the collagen production of fibroblasts from pelvic organ prolapse  

作　　者：Lei-Mei Xu Xin-Xin Yu Ning Zhang Yi-Song Chen 

机构地区：[1]Department of Gynecology,Obstetrics and Gynecology Hospital of Fudan University,Shanghai 200011,China [2]Department of Gynecology,Shanghai Key Laboratory of Female Reproductive Endocrine-related Diseases,Shanghai 200011,China

出　　处：《World Journal of Stem Cells》2024年第6期708-727,共20页世界干细胞杂志（英文版）（电子版）

基　　金：Supported by the National Natural Science Foundation of China,No.81671439;the Science and Technology Commission of Shanghai Municipality,No.21Y11906700 and No.20Y11907300;the Medical Innovation Research Project of the Science and Technology Commission of Shanghai Municipality,No.22Y11906500。

摘　　要：BACKGROUND Pelvic organ prolapse(POP)involves pelvic organ herniation into the vagina due to pelvic floor tissue laxity,and vaginal structure is an essential factor.In POP,the vaginal walls exhibit abnormal collagen distribution and decreased fibroblast levels and functions.The intricate etiology of POP and the prohibition of trans-vaginal meshes in pelvic reconstruction surgery present challenges in targeted therapy development.Human umbilical cord mesenchymal stromal cells(hucMSCs)present limitations,but their exosomes(hucMSC-Exo)are promising therapeutic tools for promoting fibroblast proliferation and extracellular matrix remodeling.suppressed inflammation in POP group fibroblasts,stimulated primary fibroblast growth,and elevated collagen I(Col1)production in vitro.High-throughput RNA-seq of fibroblasts treated with hucMSC-Exo and miRNA sequencing of hucMSC-Exo revealed that abundant exosomal miRNAs downregulated matrix metalloproteinase 11(MMP11)expression.CONCLUSION HucMSC-Exo normalized the growth and function of primary fibroblasts from patients with POP by promoting cell growth and Col1 expression in vitro.Abundant miRNAs in hucMSC-Exo targeted and downregulated MMP11 expression.HucMSC-Exo-based therapy may be ideal for safely and effectively treating POP.

关 键 词：Pelvic organ prolapse EXOSOMES FIBROBLASTS Human umbilical cord mesenchymal stromal cells Extracellular matrix Collagen I 

分 类 号：R71[医药卫生—妇产科学]