凝结芽胞杆菌发酵优化工艺  

Optimizations of fermentation of Bacillus coagulans

作　　者：何俊峰 鲁佳康 陈雄 王志 HE Jun-feng;LU Jia-kang;CHEN Xiong;WANG Zhi(School of Biological Engineering and Food/Key Laboratory of Fermentation Engineering(Ministry of Education),Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学生物工程与食品学院/发酵工程教育部重点实验室,武汉430068

出　　处：《湖北农业科学》2024年第7期147-153,共7页Hubei Agricultural Sciences

摘　　要：为提高凝结芽胞杆菌(Bacillus coagulans)的生长效率,在摇瓶、发酵罐水平考察了最适的碳源、温度、维生素添加量、pH以及发酵过程存在的氧化胁迫对凝结芽胞杆菌生长代谢的影响,获得了发酵培养基配方,并确定了30 L发酵罐最适培养条件:培养温度为40℃,初始pH为6.2~6.5,培养过程中9~14 h用氨水溶液控pH=5.5,pH大于5.5后停止控制,全程以4.1 mg/(L·h)速率流加半胱氨酸。优化条件下,发酵13 h细胞生物量达1.9×10^(10)CFU/mL,较对照提高363.4%,细胞生长效率为1.5×10^(9) CFU/(mL·h),较对照提高305.4%。显著提高了生物量、缩短了发酵周期,为凝结芽胞杆菌工业化生产提供了重要参考。In order to improve the growth efficiency of Bacillus coagulans,the effects of the appropriate carbon source,temperature,vitamin content,pH and oxidation stress in fermentation process on the growth and metabolism of Bacillus coagulans were investigated at the shaking flask and bioreactor level,and the fermentation medium was obtained.The optimum culture conditions in 30 L bioreac-tor were as follows:fermentation temperature was at 40℃,initial pH was 6.2~6.5,pH was controlled at 5.5 via the automatic addition of ammonia solution at 9~14 h during the culture process,control was stopped after pH above 5.5,and cysteine was added at a rate of 4.1 mg/(L·h)during the whole process.Under these conditions,the fermentation biomass reached 1.9×10^(10) CFU/mL at 13 h,which was 363.4%higher than that of the control,and the cell growth efficiency was 1.5×10^(9) CFU/(mL·h),which was 305.4%higher than that of the control.It significantly increased the biomass and shortened the fermentation period,which provided an important reference for the industrial production of Bacillus coagulans.

关 键 词：凝结芽胞杆菌(Bacillus coagulans) 分批发酵 培养条件 氧化应激 

分 类 号：TQ927[轻工技术与工程—发酵工程]