超声辅助低温碱提木聚糖及制备低聚木糖  

Ultrasound-assisted low-temperature alkali extraction of xylan and xylo-oligosaccharides preparation

作　　者：顾翰琦[1,2] 彭艳芳[1,2] 苏亿曼 刘晓光[1,2] 李维维 马岳杉 GU Hanqi;PENG Yanfang;SU Yiman;LIU Xiaoguang;LI Weiwei;MA Yueshan(College of Biology and Food Science,Hebei Normal University for Nationalities,Chengde 067000;Key Laboratory of Botany,State Ethnic Affairs Commission,Hebei Normal University for Nationalities,Chengde 067000)

机构地区：[1]河北民族师范学院生物与食品科学学院,承德067000 [2]河北民族师范学院植物学国家民委重点实验室,承德067600

出　　处：《中国食品添加剂》2024年第7期62-71,共10页China Food Additives

基　　金：河北省科技厅重点研发计划项目(22322803D);河北省引进留学人员资助项目(C20220301);承德市科技局“汇智领创空间”项目(HZLC2021001);河北民族师范学院重大招标项目(ZD2020001);承德市清洁能源(双碳)产业研究院(202205B090)。

摘　　要：以酒糟为原料,研究超声波辅助碱法对提取木聚糖影响,并考察提取工艺对木聚糖酶制备低聚木糖的影响。响应面实验优化超声波辅助碱提木聚糖,结果表明:在NaOH浓度8%、料液比1∶20、提取温度58℃、超声波功率955W和提取时间20 min条件下,木聚糖提取率为72.31%。通过液相色谱、凝胶色谱和红外光谱分析表明:提取木聚糖样品主要成分为木聚糖,超声处理降低相对分子量和聚合度。酶解制备低聚木糖(XOS),在酶用量为100U/g底物、pH 8.0、50℃时,酶解6 h得率达80%以上,XOS中木二糖-木四糖的含量总和(XOS2-4)占64.5%。XOS为2 mg/mL时,对DPPH自由基清除率为87.3%。本研究通过超声辅助碱溶液降低木聚糖提取能耗和木聚糖相对分子量,有效提高XOS酶解得率。该工艺将有助于提高酿酒副产物酒糟的附加值。Using vinasse as the raw material,the effects of ultrasound-assisted alkaline extraction on xylan extraction and the effects of the extraction process on the preparation of xylooligosaccharides by xylanase were studied.The response surface methodology indicated an optimal xylan yield of 72.31%was obtained under the conditions of 8%NaOH,a solid-to-liquid ratio of 1∶20,at extraction temperature of 58℃,and ultrasonic power of 955 W,and extraction time of 20 minutes.Analysis through liquid chromatography,gel chromatography,and infrared spectroscopy indicated that the main component of the extracted xylan samples was xylan,with ultrasound treatment reducing the relative molecular weight and polymerization degree.Enzymatic preparation of xylooligosaccharides(XOS)was achieved with an enzyme dosage of 100 U/g substrate,pH 8.0,and 50℃,resulting in a yield of over 80% after 6 hours of enzymatic hydrolysis,with XOS2-4(total xylobiose to xylotetraose)content constituting 64.5%.The DPPH free radical scavenging rates reached 87.3% at 2 mg/ml XOS concentration.The ultrasonic-assisted method not only reduced the extraction temperature and reaction time but also decreased molecular weight of xylan,enhancing the enzymic hydrolysis yield of XOS.This innovative process can enhance the added value of distiller's grains,a brewing by-product.

关 键 词：酒糟木聚糖 超声波辅助提取 响应面 低聚木糖 抗氧化性 

分 类 号：TQ35[化学工程] TS202.3[轻工技术与工程—食品科学]