Chemical derivatization strategies for enhancing the HPLC analytical performance of natural active triterpenoids  

作　　者：Xiao-Feng Huang Ying Xue Li Yong Tian-Tian Wang Pei Luo Lin-Sen Qing 

机构地区：[1]Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu,610041,China [2]State Key Laboratory for Quality Research in Chinese Medicines,Macao University of Science and Technology,Macao,China [3]Sichuan Center for Disease Control and Prevention,Chengdu,610041,China [4]University of Chinese Academy of Sciences,Beijing,100049,China

出　　处：《Journal of Pharmaceutical Analysis》2024年第3期295-307,共13页药物分析学报（英文版）

基　　金：Sichuan Science and Technology Program(Grant No.:2022ZYD0026);Biological Resources Program,Chinese Academy of Sciences(Grant No.:KFJ-BRP-008-007);the Macao Science and Technology Development Fund(Grant No.:0028/2019/AGJ).

摘　　要：Triterpenoids widely exist in nature,displaying a variety of pharmacological activities.Determining triterpenoids in different matrices,especially in biological samples holds great significance.High-performance liquid chromatography(HPLC)has become the predominant method for triterpenoids analysis due to its exceptional analytical performance.However,due to the structural similarities among botanical samples,achieving effective separation of each triterpenoid proves challenging,necessitating significant improvements in analytical methods.Additionally,triterpenoids are characterized by a lack of ultraviolet(UV)absorption groups and chromophores,along with low ionization efficiency in mass spectrometry.Consequently,routine HPLC analysis suffers from poor sensitivity.Chemical derivatization emerges as an indispensable technique in HPLC analysis to enhance its performance.Considering the structural characteristics of triterpenoids,various derivatization reagents such as acid chlorides,rhodamines,isocyanates,sulfonic esters,and amines have been employed for the derivatization analysis of triterpenoids.This review comprehensively summarized the research progress made in derivatization strategies for HPLC detection of triterpenoids.Moreover,the limitations and challenges encountered in previous studies are discussed,and future research directions are proposed to develop more effective derivatization methods.

关 键 词：TRITERPENOID HPLC DERIVATIZATION Quantification 

分 类 号：R446[医药卫生—诊断学]