药食用真菌珍珠发酵液对LPS诱导HaCaT炎症损伤的抵抗作用  

Anti-inflammatory Effects of Medical Edible Fungus Pearl Fermentation Broths on LPS-Induced Damage to HaCaT Cells

作　　者：蔡松雨 李泽亚 张佳婵[1,2,3,4] 阮华君 陶俊俊 王昌涛[1,2,3,4] CAI Songyu;LI Zeya;ZHANG Jiachan;RUAN Huajun;TAO Junjun;WANG Changtao(Beijing Key Laboratory of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100048;Key Laboratory of Cosmetic,China National Light Industry,Beijing Technology and Business University,Beijing 100048;Institute of Cosmetic Regulatory Science,Beijing Technology and Business University,Beijing 100048;College of Light Industry and Engineering,Beijing Technology and Business University,Beijing 100048;Zhejiang Fenix Health Science and Technology Co.,Ltd.,Zhuji 311804)

机构地区：[1]北京工商大学北京市植物资源研究开发重点实验室,北京100048 [2]北京工商大学中国轻工业化妆品重点实验室,北京100048 [3]北京工商大学化妆品监管科学研究院,北京100048 [4]北京工商大学轻工科学与工程学院,北京100048 [5]浙江长生鸟健康科技股份有限公司,诸暨311804

出　　处：《食品工业》2024年第6期124-130,共7页The Food Industry

基　　金：浙江省领军型创新创业团队项目(2020R01018);浙江省科技计划项目(2022C02037);绍兴市“揭榜挂帅”制科技项目(2021B42001)。

摘　　要：珍珠,因其卓越的保健功效而被列入可用于保健食品的中药名单。乳酸菌/酵母菌介导的珍珠发酵技术在药食妆领域已占据一席地位,但鲜有人将药食用真菌应用于珍珠的深加工利用领域。选取2种药食用真菌木蹄层孔菌(Fomes fomentarius,FF)和鲍姆木层孔菌(Phellinus baumii,PB),分别制备药食用真菌珍珠发酵液,以脂多糖(lipopolysaccharide,LPS)诱导的永生化人表皮细胞(HaCaT)为研究载体探究其潜在的抗炎能力。结果表明:较低浓度的FF和PB发酵液及其珍珠发酵液具有细胞增殖作用;相较模型组,两菌种发酵液在一定程度上可以显著降低部分炎性因子含量,珍珠粉的加入能够更有效降低炎性指标的分泌,具有抗炎功效。其中,FF珍珠发酵液在减少IL-8、IL-1β和KLK7含量上效果更优。此外,透皮吸收试验表明,PB珍珠发酵液的透皮吸收作用更佳,表示更有助于皮肤吸收。由此可见,将药食用真菌木蹄层孔菌和鲍姆木层孔菌制备珍珠发酵液,更能够发挥其抗炎作用。试验拓宽珍珠发酵菌种的范围,为珍珠的深加工利用提供一定理论依据和数据支撑,具有良好的应用价值。Due to its excellent health benefits,pearls are included in the list of herbs that can be used in health food.Lactic acid bacteria or yeasts mediated pearls fermentation has taken its place in the field of medicinal and cosmetic pearls,but few have applied medicinal fungi to the further processing and utilization of pearls.For this purpose,two medicinal edible fungi of Fomes fomentarius(FF)and Phellinus baumii(PB),were selected to prepare fermentation broths of pearl,respectively.Based on the analysis of their composition,the potential anti-inflammatory capacities of fermentation broths were investigated using lipopolysaccharide(LPS)-induced HaCaT cells.FF and PB fermentation broths at low concentrations showed cell proliferation effects.Compared with the model group,the fermentation broth of the two strains could significantly reduce the content of inflammatory factors to a certain extent,and the addition of pearl powder could more effectively reduce the secretion of inflammatory indicators,which had anti-inflammatory effect.Among them,FF pearl fermentation solution had better effect on reducing the contents of IL-8,IL-1βand KLK7.The transdermal absorption efficiency of fermentation broths was subsequently analyzed and determined.Transdermal absorption tests showed better transdermal absorption of PB pearl fermentation broth,which indicated better absorption into the skin.It can be seen that the preparation of pearl fermentation liquid by the medicinal and edible fungi Fomes fomentarius(FF)and Phellinus baumii(PB)can better exert its antiinflammatory effect.This project broadens the range of pearl fermentation strains,provides a certain theoretical basis and data support for the deep processing and utilization of pearls,and has good application value.

关 键 词：珍珠 发酵 抗炎功效 木蹄层孔菌 鲍姆木层孔菌 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ658[轻工技术与工程—食品科学与工程] TQ920.6[化学工程—精细化工]