2020-2022年郑州市食源性疾病沙门菌分子流行病学特征  

Molecular epidemiological characteristics of Salmonella isolated from the foodborne disease surveillance in Zhengzhou city from 2020 to 2022

作　　者：兰培利 戴蕾[1] 卫少华 赵瑞臻[1] 周鹏 程春荣[1] LAN Peili;DAI Lei;WEI Shaohua;ZHAO Ruizhen;ZHOU Peng;CHENG Chunrong(Zhengzhou Center for Disease Contol and Prevention,Zhengzhou 450007,Henan,China)

机构地区：[1]郑州市疾病预防控制中心,河南郑州450007

出　　处：《现代疾病预防控制》2024年第6期436-441,共6页MODERN DISEASE CONTROL AND PREVENTION

基　　金：河南省医学科技攻关计划(联合共建)项目(LHGJ20230637)。

摘　　要：目的了解郑州市食源性疾病哨点医院监测中分离到的沙门菌分子流行病学特征,为食源性沙门菌的检测及相关分析提供参考。方法对2020—2022年郑州市5家哨点医院从食源性疾病病例标本中分离的沙门菌菌株进行分离鉴定及血清分型,微量肉汤稀释法进行药敏实验,并用脉冲场凝胶电泳(PFGE)对菌株进行分子分型,分析菌株之间的相关性。同时分析其人群及时间分布。结果2020—2022年5家哨点医院共分离鉴定出沙门菌192株,分离自109名男性和83名女性食源性疾病病例,年龄6月龄至73岁。0~<4岁病例分离的沙门菌菌株数占59.90%(115株),6—9月分离的菌株占64.58%(124株)。192株沙门菌分属8个血清群和28种血清型,其中肠炎沙门菌为优势血清型(79株,41.15%),鼠伤寒沙门菌次之(55株,28.65%);0~<4岁病例分离的沙门菌中鼠伤寒沙门菌是优势血清型(35.65%,41/115)。沙门菌对亚胺硫霉素最敏感(99.48%),对氨苄西林耐药率最高(88.02%);耐3种及以上抗生素的菌株占83.85%。79株肠炎沙门菌分属26种PFGE带型,相似度为51.84%~100.00%,有11组完全相同的PFGE图谱。结论2020—2022年郑州市食源性疾病监测沙门菌在低幼人群与其他人群优势血清型不同,相关部门应结合其分子流行病学特征加强监控。菌株的耐药性需引起医疗机构关注,并重视抗生素的规范使用。Objective Understanding the molecular epidemiological characteristics of Salmonella isolated from foodborne diseases surveillance in sentinel hospitals in Zhengzhou,providing reference for the detection and related analysis of foodborne Salmonella.Methods From 2020 to 2022,Salmonella isolation submitted from the five sentinel hospitals in Zhengzhou city were identified and serotyped.The drug sensitivity test was carried out by micro-broth dilution method.The molecular typing of the strains was performed by pulsed field gel electrophoresis(PFGE)to analyze the correlation among the strains.Meanwhile,the population and time distribution were analyzed.Results The population and time distribution analysis showed the 192Salmonella were identified from the patients including 109 males and 83 females,and the age of patients ranged from 6 month to73 years.The proportion of patients under 4 years old was highest(59.90%,115)among all age groups.The majority of salmonella infections occurred between June and September(64.58%,124).The laboratory test results showed that eight serogroups and 28 serotypes were identified among 192 Salmonella isolates,with S.enteritidis(41.15%,79)as the first dominant serotypes,followed by S.typhimurium(28.65%,55).S.typhimurium was the dominant serotype(35.65%,41/115)among Salmonella isolated from cases aged 0 to<4 years old.The most of Salmonella isolates were sensitive to iminothiomycin(99.48%),and the most of them were resistant to ampicillin(88.02%).The proportion of the isolates resistant to at least three antibiotics accounted for83.85%.There were 26 PFGE types of the 79 strains of S.enteritidis,with the similarity of 51.84%-100.00%,and 11 pairs of identical PFGE patterns were observed.Conclusions Of the Salmonella isolated from food-borne disease surveillance in Zhengzhou city from 2020 to 2022,the dominant serotype among the young population was different from that among the other population,the relevant departments should strengthen monitoring based on their molecular epidemiological ch

关 键 词：食源性 沙门菌 血清分型 耐药性 脉冲场凝胶电泳 

分 类 号：R155.3[医药卫生—营养与食品卫生学] R181.3[医药卫生—公共卫生与预防医学]