Dynamic modulation of nodulation factor receptor levels by phosphorylation-mediated functional switch of a RING-type E3 ligase during legume nodulation  

作　　者：Hao Li Yajuan Ou Jidan Zhang Kui Huang Ping Wu Xiaoli Guo Hui Zhu Yangrong Cao 

机构地区：[1]National Key Lab of Agricultural Microbiology,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,China [2]National Key Lab of Agricultural Microbiology,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China

出　　处：《Molecular Plant》2024年第7期1090-1109,共20页分子植物（英文版）

基　　金：the National Key R&D Program of China(2019YFA0904700);the National Natural Science Foundation of China(32200207 and 32090063);the China Postdoctoral Science Foundation(2019M662652);a Self-lnnovation grant from National Laboratory(AML2023B01).

摘　　要：The precise control of receptor levels is crucial for initiating cellular signaling transduction in response to specific ligands;however,such mechanisms regulating nodulation factor(NF)receptor(NFR)-mediated perception of NFs to establish symbiosis remain unclear.In this study,we unveil the pivotal role of the NFR-interacting RING-type E3 ligase 1(NIRE1)in regulating NFR1/NFR5 homeostasis to optimize rhizobial infection and nodule development in Lotus japonicus.We demonstrated that NiRE1 has a dual function in this regulatory process.It associates with both NFR1 and NFR5,facilitating their degradation through K48-linked polyubiquitination before rhizobial inoculation.However,following rhizobial inoculation,NFR1 phosphorylates NIRE1ata conserved residue,Tyr-109,inducing a functional switch in NIRE1,which enables NIRE1tomediateK63-linkedpolyubiquitination,thereby stabilizing NFR1/NFR5 in infected root cells.The introduction of phospho-dead NIRE1Y1osF leads to delayed nodule development,underscoring the significance of phosphorylation at Tyr-1o9 in orchestrating symbiotic processes.Conversely,expression of the phospho-mimic NIRE1Y0E results in the formation of spontaneous nodules in L.japonicus,further emphasizing the critical role of the phosphorylation-dependent functional switch in NiRE1.In summary,these findings uncover a fine-tuned symbiotic mechanism that a single E3 ligase could undergo a phosphorylationdependent functional switch to dynamically and precisely regulate NF receptor protein levels.

关 键 词：nodule symbiosis nodulation factor receptors E3 ligase UBIQUITINATION PHOSPHORYLATION protein degradation protein stabilization Lotus japonicus 

分 类 号：Q94[生物学—植物学]