EMC6与Apaf-1、Caspase-3在宫颈癌组织中的表达及临床预后意义  

Expression and clinical prognosis of EMC6,Apaf-1 and Caspase-3 in cervical cancer tissues

作　　者：苗春霞 张云霞[1] 王一娜[1] 申明霞 MIAO Chunxia;ZHANG Yunxia;WANG Yina;SHEN Mingxia(Gynecological Radiotherapy First Ward,the Affiliated Tumor Hospital of Xinjiang Medical University,Urumqi 830011,China;Xinjiang Uygur Autonomous Region Hospital of Traditional Chinese Medicine,Urumqi 830000,China)

机构地区：[1]新疆医科大学附属肿瘤医院妇科放疗一病区,乌鲁木齐830011 [2]新疆维吾尔自治区中医医院,乌鲁木齐830000

出　　处：《新疆医科大学学报》2024年第7期920-924,共5页Journal of Xinjiang Medical University

基　　金：国家自然科学基金项目(81960472)。

摘　　要：目的 探讨宫颈癌中EMC6、Apaf-1和Caspase-3蛋白的表达情况,并分析其与患者临床病理特征和预后的关系。方法 采用免疫组化方法检测宫颈癌及癌旁组织中EMC6、Apaf-1和Caspase-3蛋白的表达,并收集患者的临床病理资料进行分析,Spearman检测变量间的相关性,单因素及多因素Cox分析预后影响因素。结果 EMC6、Apaf-1及Caspase-3蛋白在宫颈癌组织中的阳性表达率均低于癌旁组织,差异有统计学意义(P均<0.05);不同临床病理特征间EMC6蛋白阳性表达率差异无统计学意义(P>0.05);不同分化程度宫颈癌患者中Apaf-1蛋白阳性表达率差异有统计学意义(χ^(2)=4.723,P=0.030);不同临床分期和分化程度宫颈癌患者中Caspase-3蛋白阳性表达率差异有统计学意义(χ^(2)=4.495,P=0.034;χ^(2)=5.010,P=0.025)。EMC6与Apaf-1、Caspase-3相关性系数分别为r=0.626(P<0.001)、r=0.554(P<0.001),均呈正相关;单因素检验得出,病理类型(P=0.047)、肿瘤大小(P<0.001)、EMC6(P=0.004)、Apaf-1(P=0.029)和Caspase-3(P=0.039)是影响宫颈癌患者总体生存期的预后因素;多因素分析显示,EMC6(P=0.005)、肿瘤大小(P<0.001)是宫颈癌患者不良预后的独立影响因素。结论 宫颈癌组织中EMC6、Apaf-1和Caspase-3的低表达与患者预后不良相关,其中EMC6与Apaf-1、Caspase-3呈正相关,且EMC6和肿瘤大小是独立的预后因素,这些蛋白在宫颈癌进展中发挥重要作用,有望成为预后标志物和治疗靶点。Objective To investigate the expression of EMC6,Apaf-1 and Caspase-3 proteins in cervical cancer and to analyze their correlation with the clinical-pathological characteristics and prognosis of the patients.Methods Immunohistochemical method was used to detect the expression of EMC6,Apaf-1 and Caspase-3 proteins in cervical cancer and adjacent non-cancerous tissues.Clinical-pathological data of the patients were collected for analysis.Spearman was used to detect the correlation between variables.Univariate and multivariate Cox regression analyses were conducted to identify prognostic factors.Results The positive expression rates of EMC6,Apaf-1 and Caspase-3 proteins in cervical cancer tissues were significantly lower than those in adjacent tissues(P<0.05).There was no significant difference in the positive expression of EMC6 protein among different clinicopathological features(P>0.05).The positive expression of Apaf-1 protein in patients with different differentiation degrees of cervical cancer was significantly different(χ^(2)=4.723,P=0.030).The positive expression of Caspase-3 protein in cervical cancer patients with different clinical stages and differentiation degrees was significantly different(χ^(2)=4.495,P=0.034;χ^(2)=5.010,P=0.025).The correlation coefficients between EMC6 and Apaf-1,as well as between EMC6 and Caspase-3,were 0.626(P<0.001) and 0.554(P<0.001),respectively,indicating a positive correlation.Univariate test showed that pathological type(P=0.047),tumor size(P<0.001),EMC6(P=0.004),Apaf-1(P=0.029) and Caspase-3(P=0.039) were prognostic factors affecting the overall survival of patients with cervical cancer.Multivariate analysis showed that EMC6(P=0.005) and tumor size(P<0.001) were independent influencing factors of poor prognosis in patients with cervical cancer.Conclusion In cervical cancer tissues,low expression of EMC6,Apaf-1 and Caspase-3 is associated with poor patient prognosis.EMC6 is positively correlated with Apaf-1 and Caspase-3,and both EMC6 and tumor size are independent prognos

关 键 词：EMC6 APAF-1 CASPASE-3 宫颈癌 预后 凋亡 协同作用 

分 类 号：R737.33[医药卫生—肿瘤]