虚拟模板MIPs-SPE联用技术对茶叶粗提物中咖啡因的分离纯化研究  

Study on the separation and purification of Caffeine from tea extract by virtual template MIPs SPE combined technology

作　　者：何倩[1,2] 孙亚坤[3] 程煜凤 杨建[1] 王超[1] HE Qian;SUN Yakun;CHENG Yufeng;YANG Jian;WANG Chao(College of Pharmacy,Xinjiang Medical University,Urumqi 830017,China;Department of Pharmacy,Affiliated Hospital of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830002,China;College of Pharmacy,Shihezi University,Shihezi Xinjiang 832000,China)

机构地区：[1]新疆医科大学药学院,乌鲁木齐830017 [2]新疆医科大学附属中医医院药学部,乌鲁木齐830002 [3]石河子大学药学院,新疆石河子832000

出　　处：《新疆医科大学学报》2024年第7期1014-1018,共5页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区重大科技专项子课题(2022A03018-4);新疆天然药物活性组分与释药技术重点实验室开放课题(XJDX1713)。

摘　　要：目的 采用儿茶素作为虚拟模板制备分子印迹聚合物(Molecular imprintingpolymers,MIPs)结合固相萃取(Solid phase extraction,SPE)技术对茶叶粗提物中的咖啡因进行分离纯化。方法 以儿茶素为虚拟模板,4-乙烯基吡啶为功能单体,乙二醇二甲基丙烯酸酯为交联剂,聚苯乙烯为大分子拥挤试剂,以原位聚合的方式制备虚拟模板分子印迹聚合物。测定该分子印迹聚合物的保留性能、形貌特征和孔径分布等性能,以该分子印迹聚合物作为固相萃取吸附剂,对茶叶粗提物中的咖啡因进行分离纯化。结果 所制备儿茶素分子印迹聚合物对咖啡因的最优印迹因子为4.57。对茶叶粗提物中的咖啡因进行分离纯化后,在咖啡因纯度高于95%的条件下,咖啡因的回收率可达85%以上。结论 以儿茶素为虚拟模板合成的分子印迹聚合物,可以用于茶叶粗提物中咖啡因的分离纯化。Objective Catechins were usedas virtual templates to preparemolecular imprinting polymers(MIPs) and solid-phase extraction(SPE) technology to separate and purify caffeine from crude tea extract.Methods A virtual template molecularly imprinted polymer was synthesized by in-situ polymerization using catechins as virtual template molecules,4-vinylpyridine as functional monomer,ethylene glycol dimethacrylate as crosslinking agent and polystyrene as macromolecular crowding reagent.On the basis of characterizing the imprinted polymer,it was used as solid phase extraction adsorbent to separate and purify caffeine from crude tea extracts.Results The optimal imprinting factor for caffeine in the prepared catechin molecularly imprinted polymer was 4.57.After separating and purifying caffeine from crude tea extracts,the recovery rate of caffeine can reach over 85% under the condition of ensuring caffeine purity above 95%.Conclusion The molecularly imprinted polymer synthesized using catechin as a virtual template can be applied for the separation and purification of caffeine from tea extracts.

关 键 词：虚拟模板 分子印迹聚合物 固相萃取 儿茶素 咖啡因 分离纯化 

分 类 号：R917[医药卫生—药物分析学]