Investigation of the Cytotoxicity of CAR-NK-92 Cells Targeting CEA Against Gastric Cancer Cells  

作　　者：Xinyu Zheng Xiaoxiao Zhou Xingzhou Xia Xiaomeng Chen 

机构地区：[1]Department of Gastroenterology,The Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China

出　　处：《Journal of Clinical and Nursing Research》2024年第6期410-416,共7页临床护理研究（英文）

基　　金：Henan Provincial Health Commission's 2020 National Health Commission Science Research Fund Henan Provincial Medical Science and Technology Tackling Plan Provincial-Ministerial Joint Project and Soft Science Project"Clinical Study of CAR-NK Cells Targeting Carcinoembryonic Antigen on Gastric Cancer Cells"(Grant No.SBGJ202002093);Henan Province 2022 Science and Technology Development Plan"Study on Pyroglutamate Targeting DJ-1 to Trigger ROS-Induced Cell Death and Protective Autophagy in Pancreatic Cancer"(Grant No.222102310725)。

摘　　要：Objective:To construct CAR-NK-92 cells targeting carcinoembryonic antigen(CEA)and study their killing effect on gastric cancer cells.Methods:CAR-NK-92 cells targeting CEA were constructed.After co-culturing CAR-NK-92 cells with MKN-45 gastric cancer cells,the killing effect of CAR-NK-92 cells was detected by a lactate dehydrogenase release assay.The secretion levels of gamma interferon and granulocyte-macrophage colony-stimulating factor were measured using an ELISA assay.Results:The lactate dehydrogenase release assay showed that CAR-NK-92 cells had a significant killing effect on MKN-45 cells compared to CON-NK-92 cells,and the difference was statistically significant(P<0.001).ELISA results indicated that the levels of gamma interferon and granulocyte-macrophage colony-stimulating factor secreted by CAR-NK-92 cells and MKN-45 target cells were significantly increased after co-culture(P<0.001).Conclusion:CAR-NK-92 cells targeting CEA exhibit a significant killing effect on CEA-positive gastric cancer cells.

关 键 词：Gastric cancer Natural killer cell Carcinoembryonic antigen IMMUNOTHERAPY 

分 类 号：R73[医药卫生—肿瘤]