新型食品添加剂吡咯喹啉醌的分离纯化工艺研究  

Research on the Isolation and Purification Process of Novel Food Additive PQQ

作　　者：杜海军 徐慧丽 DU Haijun;XU Huili(Zhejiang Laiyi Biotechnology Co.,Ltd.,Shaoxing 312400,China;Zhejiang Pharmaceutical Co.,Ltd.,Xinchang Pharmaceutical Factory,Shaoxing 312500,China)

机构地区：[1]浙江来益生物技术有限公司,浙江绍兴312400 [2]浙江医药股份有限公司新昌制药厂,浙江绍兴312500

出　　处：《食品安全导刊》2024年第14期74-76,80,共4页China Food Safety Magazine

摘　　要：为了对新食品添加剂吡咯喹啉醌(Pyrroloquinoline Quinone,PQQ)发酵上清液分离纯化工艺中大孔树脂吸附洗脱步骤进行优化,提升洗脱收率,本文采用XAD-1600大孔树脂对上柱液中PQQ进行吸附和洗脱,对洗脱剂种类进行筛选,对上柱液的pH值、洗脱剂中氯化钠的浓度、洗脱温度和洗脱流速等工艺参数进行优化,计算洗脱收率。结果表明,在PQQ上柱液pH值为3.5,洗脱剂选择磷酸盐缓冲液,磷酸盐缓冲液中氯化钠含量为1.4%,洗脱流速为2 BV·h^(-1),洗脱温度为20℃的条件下,PQQ洗脱收率最高(96.7%)。In order to optimize the adsorption and elution steps of macroporous resin in the separation and purification process of the new food additive pyrroloquinoline quinone(PQQ)fermentation supernant and improve the elution yield,XAD-1600 macroporous resin was used to adsorb and eluate PQQ in the upper column liquid.The elution yield was calculated by optimizing the pH value of the upper column liquid,the concentration of sodium chloride in the eluent,the elution temperature and the elution flow rate.The results showed that under the conditions of pH value of PQQ upper column solution was 3.5,phosphate buffer was selected as elution agent,sodium chloride content in phosphate buffer was 1.4%,elution flow rate was 2 BV·h^(-1),and elution temperature was 20℃,the elution yield of PQQ was the highest(96.7%).

关 键 词：新食品添加剂 吡咯喹啉醌 分离纯化 大孔树脂 洗脱收率 

分 类 号：TS202.3[轻工技术与工程—食品科学]