离子色谱指纹图谱分析在烟用香精香料品质评价中的应用  

Application of ion chromatographic fingerprint analysis for quality evaluation of tobacco flavors

作　　者：许高燕[1] 何歆滢 张丽娜[1] 刘崇盛 高阳[1] 黄忠平 刘会君[2] 吴兆明[1] 章瑞潮 施宏 XU Gaoyan;HE Xinying;ZHANG Lina;LIU Chongsheng;GAO Yang1;HUANG Zhongping;LIU Huijun;WU Zhaoming;ZHANG Ruichao;SHI Hong(Technical Center,China Tobacco Zhejiang Industry Co.,Ltd.,Hangzhou 310008,China;College of Chemical Engineering,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江中烟工业有限责任公司技术中心,浙江杭州310008 [2]浙江工业大学化学工程学院,浙江杭州310014

出　　处：《色谱》2024年第8期805-811,共7页Chinese Journal of Chromatography

基　　金：浙江中烟工业有限责任公司科技项目(ZJZY2022C001)。

摘　　要：为保证烟用香精香料品质的一致性,采用离子色谱法对烟用香精香料样品中的9种有机酸及7种无机阴离子进行分析。1.0 g样品中加入10 mL去离子水,振荡萃取30 min后,水相溶液用0.45μm微孔滤膜过滤,滤液进一步采用RP前处理柱除去基质,经离子色谱仪分离测定。采用Dionex IonPac AS11-HC阴离子交换柱及淋洗液梯度洗脱,9种有机酸和7种常规无机阴离子标准工作溶液的保留时间和峰面积日内精密度(RSD)分别为0.01%~0.69%和1.34%~2.98%,日间RSD分别为0.03%~0.68%和3.54%~5.16%。同时对烟用香精香料标准样品A~D(各5个批次)中有机酸和无机酸的保留时间和峰面积RSD进行考察,得到的保留时间和峰面积日内RSD分别为0.01%~0.71%和2.39%~3.22%,日间RSD分别0.05%~0.81%和3.61%~6.02%。建立了4种烟用香精香料(各5个批次)的指纹谱图库,并采用系统聚类分析和相似度分析法对其他生产厂家样品进行品质评价。结果表明,聚类分析法能有效地从不同生产厂家的产品中筛选出与标准样品质量最相近的样品;实际样品AY3、BY2、CY2及DY1与香精香料标准品性质最接近,品质较好。相似度分析可以对不同生产厂家的产品质量进行更加具体、量化的评价,实际样品AY3、BY2、CY2及DY1与香精香料标准品的化学成分相似度值均大于97.7%,符合相似度评价要求。相较于超声辅助液液萃取-气相色谱法,对于个别特殊样品,离子色谱法能更有效地区分香精香料样品间的品质差异。Tobacco flavor,an important tobacco additive,is an essential raw material in cigarette production that can effectively improve the quality of tobacco products,add aroma and taste,and increase the suction flavor.The quality consistency of tobacco flavors affects the quality stability of branded cigarettes.Therefore,the quality control of tobacco flavors is a major concern for cigarette and flavor manufacturers.Physical and chemical indices,odor similarity,and sensory efficacy are employed to evaluate the quality of tobacco flavors,and the analysis of chemical components in tobacco flavors is usually conducted using gas chromatography(GC)and high performance liquid chromatography(HPLC).However,because the composition of tobacco flavors is complex,their quality cannot be fully reflected using a single component or combination of components.Therefore,establishing an objective analytical method for the quality control of tobacco flavors is of extreme importance.Chromatographic fingerprint analysis is routinely used for the discriminative analysis of tobacco flavors.Chromatographic fingerprints refer to the general characteristics of the concentration profiles of different chemical compounds.In the daily procurement process,fingerprints established by GC and HPLC are effective for the evaluation and identification of tobacco flavors.However,given continuous improvements in aroma-imitation technology,some flavors with high similarity cannot be directly distinguished using existing methods.

关 键 词：离子色谱法 烟用香精香料 色谱指纹图谱 聚类分析 相似度分析 

分 类 号：O658[理学—分析化学]