Failure of Cronobacter sakazakii to acquire direct and cross-protection against lethal stresses after exposure to sublethal ethanol  

作　　者：Hongmei Niu Xiang Wang Yuan Wang Xiaojie Qin Yangtai Liu Zhuosi Li Xu Wang Wantong Zhang Qingli Dong 

机构地区：[1]School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai,China [2]School of Food and Drugs,Shanghai Zhongqiao Vocational and Technical University,Shanghai,China [3]Yili Group Innovation Centre,Shanghai,China [4]Yili Yinuo Technology(Shanghai)Co.,Ltd.,Shanghai,China

出　　处：《Food Quality and Safety》2024年第1期81-91,共11页食品品质与安全研究（英文版）

基　　金：the Shanghai Project of Food Safety Risk Assessment(RA-2022-10);the Shanghai Agriculture Applied Technology Development Program(2022-02-08-00-12-F01144),China.

摘　　要：Cronobacter sakazakii may encounter subinhibitory concentrations of ethanol stress over its lifecycle.Bacterial tolerance to homologous or heterologous stress agents may be altered as a result of ethanol adaptive responses.Therefore,the tolerance of ethanol-exposed and control cells to subsequent lethal stresses was evaluated in the current work.It was discovered that sublethal ethanol exposure increased the susceptibility to lethal ethanol stress in C.sakazakii as determined by the Weibull model.Furthermore,sublethal ethanol concentration exposure in C.sakazakii did not lead to any cross-tolerance against other stressors such as benzalkonium chloride(120 mg/L),heat(55°C),cold(4°C),simulated gastric fuid(pH 3.0),osmotic stress(sorbitol,0.75 g/mL),and desiccation stress.Analysis of zeta potential,scanning electron microscope,and surface-enhanced Raman spectroscopy spectra revealed that cellular injury and changes in cellular chemical composition may contribute to the reduced resistance of C.sakazakii after ethanol exposure.Furthermore,sublethal ethanol exposure resulted in an elevated proportion of unsaturated fatty acids(USFA),while reducing the proportion of saturated fatty acids(SFA)and the ratio of SFA to USFA.The developed inactivation models can serve as a valuable source of data to support quantitative microbial risk assessment.Moreover,a better understanding of the response of C.sakazakii to sublethal ethanol exposure may provide valuable insights into the prevention and control of C.sakazakii.

关 键 词：Cronobacter sakazakii ethanol exposure stress tolerance direct resistance CROSS-RESISTANCE mechanisms. 

分 类 号：TS201.3[轻工技术与工程—食品科学]