黄芩素对MC3T3-E1体外成骨分化及对口腔常见菌的抑菌作用  

Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria

作　　者：王越 林芳辰 苏琦 黄霞 周志迎[2] WANG Yue;LIN Fangchen;SU Qi;HUANG Xia;ZHOU Zhiying(College of Stomatology,Jinan University,Guangzhou 510632,China;Department of Orthodontics,The First Affiliated Hospital of Jinan University,Guangzhou 510630,China)

机构地区：[1]暨南大学口腔医学院,广东广州510632 [2]暨南大学附属第一医院口腔中心,广东广州510630

出　　处：《中山大学学报（医学科学版）》2024年第4期602-612,共11页Journal of Sun Yat-Sen University:Medical Sciences

摘　　要：【目的】研究不同浓度黄芩素对MCT3-E1增殖及生物学行为的影响,以及其对口腔常见菌的抑菌作用,并探讨其相关机制。【方法】将MC3T3-E1细胞分别培养在0、6、12、18、24µmol/L的黄芩素浓度下,通过CCK-8实验检测黄芩素处理后MC3T3-E1的增殖活性;对成骨诱导培养后的MC3T3-E1进行ALP活性检测;RT-PCR法检测RunX2、BMP2、Osterix基因的表达差异。K-B纸片法检测黄芩素处理24 h对大肠杆菌,金黄色葡萄球菌,血链球菌的抑菌效果。【结果】黄芩素可在一定程度降低细胞增殖活力,但不影响其继续增殖。18µmol/L的黄芩素可以提高MC3T3-E1的ALP活性,并有效上调BMP2与Osterix的表达,下调RunX2的表达,能有效抑制金黄色葡萄球菌与血链球菌的增殖(P<0.05)。【结论】适宜浓度的黄芩素(18µmol/L)对MC3T3-E1细胞成骨分化有促进作用,并且可有效抑制口腔常见菌(金黄色葡萄球菌与血链球菌)的增殖。【Objective】To investigate the impact of varying concentrations of baicalein on the proliferation and biological responses of MC3T3-E1 cells,as well as the antibacterial efficacy of baicalein against prevalent oral bacteria,and to elucidate the underlying mechanisms.【Methods】MC3T3-E1 cells were exposed to different concentrations of baicalein(0,6,12,18,and 24µmol/L)and cell viability was determined by using the CCK-8 assay.Alkaline phosphatase(ALP)activity of MC3T3-E1 cells following osteogenic induction was assessed.RT-PCR was used to examine the expression of RunX2,BMP2,and Osterix.After 24 hours of treatment,the antibacterial potential of baicalein against Escherichia coli,Staphylococcus Aureus and Streptococcus Sanguis was evaluated by using the K-B paper disk method.【Results】Baicalein exhibited a modest reduction in proliferation of MC3T3-E1 cells but without affecting their sustained proliferation.Baicalein at a concentration of 18µmol/L enhanced ALP activity of MC3T3-E1 cells,upregulated BMP2 and Osterix expression,downregulated RunX2 expression,significantly inhibited the proliferation of Staphylococcus Aureus and Streptococcus Sanguis(P<0.05).【Conclusions】Baicalein at an optimal concentration(18µmol/L)demonstrated a promotional effect on the osteogenic differentiation of MC3T3-E1 cells and effectively suppressed the proliferation of common oral bacteria,including Staphylococcus Aureus and Streptococcus Sanguis.

关 键 词：前成骨细胞 黄芩素 成骨作用 抑菌 分化 增殖 

分 类 号：R781.42[医药卫生—口腔医学]