短乳杆菌YSJ2缓解CUMS小鼠抑郁症状的研究  

Study on alleviating depressive symptoms of CUMS mice uith Lactobacillus brevis YSJ2

作　　者：姚昱锟 刘伊索 郑婷 李健坤 张太 刘琦琦 易华西[1,2] YAO Yukun;LIU Yisuo;ZHENG Ting;LI Jiankun;ZHANG Tai;LIU Qiqi;YI Huaxi(College of Food Science and Engineering,Ocean University of China,Qingdao 266000,China;Food laboratory of Zhongyuan,Luohe 462300,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266000 [2]中原食品实验室,河南漯河462300

出　　处：《中国乳品工业》2024年第7期5-10,41,共7页China Dairy Industry

基　　金：国家自然科学基金(32172180)。

摘　　要：随着“肠-脑轴”理论的发展“,精神益生菌”成为防治神经及精神类疾病的研究热点。γ-氨基丁酸(GABA)是一种抑制性神经递质,可有效缓解抑郁样行为表现。研究筛选得到1株高产GABA的短乳杆菌YSJ2(Lactobacillus brevis YSJ2),对其发酵特性和胃肠耐受性进行评价,并探究了其对慢性不可预知温和刺激(CUMS)模型小鼠的抑郁改善作用。结果表明,短乳杆菌YSJ2具有良好的发酵特性和胃肠耐受性,能提高慢性应激小鼠在旷场试验中的运动总距离,增加其在高架十字迷宫试验中的开臂运动时间百分比,减少其在强迫游泳试验和悬尾试验中的不动时间;其作用机制与减少血清中皮质酮含量,上调海马体中盐皮质激素受体表达,进而提高小鼠下丘脑-垂体-肾上腺轴兴奋性有关。此外,YSJ2还可以减轻抑郁小鼠脑组织神经元损伤,为进一步开发改善抑郁症的精神益生菌研究提供了理论依据。With the development of the"gut-brain axis"theory,"psycho-probiotics"have become a hot research topic for the prevention and treatment of neurological and psychiatric diseases.γ-aminobutyric acid(GABA)is an inhibitory neurotransmitter,which is effective in alleviating depressive behaviors.In this study,we selected a strain of Lactobacillus brevis YSJ2 with high GABA production,evaluated its fermentation characteristics and gastrointestinal tolerance,and investigated its effects on depression in mice with chronic unpredictable mild stress(CUMS)model.The results showed that Lactobacillus brevis YSJ2 had good fermentation properties and gastrointestinal tolerance,and could increase the total distance travelled by chronically stressed mice in the open-field experiment,increase the percentage of time spent in open-arm movement in the elevated cross-maze experiment,and decrease the immobility time in the forced-swimming experiment and the hanging-tail experiment.The mechanism was related to the reduction of serum corticosterone content and up-regulation of salt corticosteroid receptor expression in the hippocampus,which in turn increased the excitability of the hypothalamic-pituitary-adrenal axis in mice.In addition,YSJ2 can also reduce neuronal damage in brain tissue of depressed mice,which provides a theoretical basis for further development of psycho-probiotics to improve depression.

关 键 词：精神益生菌 短乳杆菌 抑郁 Γ-氨基丁酸 

分 类 号：TS201.3[轻工技术与工程—食品科学] R589.7[轻工技术与工程—食品科学与工程]