基于磁控溅射的PEEK表面HA涂层构建及其生物安全性评估  

Construction of HA coating on PEEK surface based on magnetron sputtering and its biosafety evaluation

作　　者：马天源 陆斌 刘富伟 辛河 陈怡成 贾雪连 朱思敏 张浚睿 宁小娜 MA Tianyuan;LU Bin;LIU Fuwei;XIN He;CHEN Yicheng;JIA Xuelian;ZHU Simin;ZHANG Junrui;NING Xiaona(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Clinical Research Center for Oral Diseases,Department of Oral and Maxillofacial Surgery,The Third Hospital Affiliated of Air Force Military Medical University,Xi'an,710032,China;Life Sciences,Northwestern University,Xi'an;Department of Ophthalmology,The Second Hospital Affiliated of Air Force Military Medical University,Xi'an)

机构地区：[1]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床医学研究中心,空军军医大学第三附属医院口腔颌面外科,西安710032 [2]西北大学生命科学学院 [3]空军军医大学第二附属医院眼科

出　　处：《实用口腔医学杂志》2024年第4期467-472,共6页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:82101065);陕西省重点研发计划(编号:2023-YBSF-112);陕西省卫生健康科研基金项目(编号:2022D032);空军军医大学第二附属医院学科创新发展计划(编号:2021ZTXM-011)。

摘　　要：目的:基于磁控溅射技术在PEEK表面构建羟基磷灰石(HA)涂层,并系统评估该方法的生物安全性。方法:磺化PEEK以增加结合面积,并利用磁控溅射技术在其上构建HA涂层;通过扫描电镜结合能谱分析检测构建效果;通过细胞黏附实验、细胞骨架荧光染色以及扫描电镜来评估细胞层面安全性;通过SD大鼠全身毒性评估材料在体安全性;通过金黄地鼠黏膜刺激实验评估材料口腔应用安全性。结果:利用上述技术可在PEEK表面成功构建具有梯度形貌的HA涂层;该涂层可促进细胞黏附、伸展与增殖;且无全身毒性,对动物体重与体内重要脏器HE染色均无显著性影响(P>0.05);同时该涂层在一定程度上减轻了单纯磺化带来的口腔黏膜刺激。结论:基于磁控溅射技术可稳定制备HA涂层,并满足临床应用的生物安全性需求。Objective:To prepare hydroxyapatite(HA)coating on polyether ether ketone(PEEK)surface by magnetron sputtering technique and to study its biosafety.Methods:Sulfonated PEEK was used to increase the binding area and HA coating was constructed on it using magnetron sputtering technology.SEM and energy dispersive spectroscopy(EDAX)were used to detect the construction effect.Cell adhesion assay,cytoskeletal fluorescence staining and SEM validation were used to assess cytologrcal safety.In vivo safety tests were conducted in SD rats and golden hamsters.Results:HA coating with gradient morphology was successfully constructed on the PEEK surface using above technique.The coating promoted cell adhesion,extension and proliferation.No systemic toxicity and no significant influence in HE staining of the main infernal organs samples were observed.The coating alleviated the oral mucosal irritation caused by simple sulfonation to a certain extent.Conclusion:HA coating can be prepared stably with magnetron sputtering technology and can meet the biosafety needs for clinical applications.

关 键 词：聚醚醚酮(PEEK) 羟基磷灰石(HA)涂层 磁控溅射 生物安全性 

分 类 号：R783.1[医药卫生—口腔医学]