江苏省内市售豆干中转基因成分的检测与分析  

Detection and analysis of genetically modified ingredients in dried bean curds in Jiangsu market

作　　者：方昕 王雯 王钰 徐春祥 吴小芹 高惠敏 FANG Xin;WANG Wen;WANG Yu;XU Chunxiang;WU Xiaoqin;GAO Huimin(College of Forestry,Nanjing Forestry University,Nanjing 210037,China;Jiangsu Institute for Food and Drug Control,Nanjing 210008,China)

机构地区：[1]南京林业大学林学院,江苏南京210037 [2]江苏省食品药品监督检验研究院,江苏南京210008

出　　处：《生物加工过程》2024年第4期440-446,共7页Chinese Journal of Bioprocess Engineering

基　　金：江苏省市场监管局科技计划(KJ2023025、KJ21125094)。

摘　　要：目前市售的豆制品大多无转基因标识,但其生产原料是否真为非转基因大豆却难以鉴别。本研究选取江苏省内120批次豆干为研究对象,以转基因大豆常用的调控元件、标记基因和目的基因等为靶标,运用实时荧光PCR技术分析豆干中是否含有转基因成分。结果表明,120份豆干中检出转基因成分的有43份,检出率为35.8%。花椰菜花叶病毒35S启动子(CaMV35S)是检出率最高的外源基因。耐草甘膦相关基因的检出率高于耐草铵膦基因。从流通环节看,江苏省13市中,宿迁的转基因豆干发现率最高,其次为南通;从生产环节看,生产于重庆的豆干转基因成分发现率最高,安徽、湖南分别位列第二、三。本研究为转基因食品的安全监管工作提供了数据支持和技术支撑。Because most soybean products in the market do not have GM labels,it is difficult to distinguish whether non-GM soybeans were used as raw materials.In this study,120 batches of dried bean curds,which were collected in Jiangsu Province as the research objects,were subjected to real time fluorescent PCR to detect GM ingredients using the regulatory elements,marker genes and target genes of transgenic soybeans as the detection targets.As a result,43 of 120 dried bean curds were found to contain GM ingredients,with a detection rate of 35.8%.Cauliflower mosaic virus 35S promoter(CaMV35S)showed the highest detection rate among all exogenous genes.In addition,glyphosate tolerance related genes displayed higher detection rate than glufosinate tolerance related genes.Among all 13 places where we collected curd samples in Jiangsu,Suqian city showed the highest detection rate of GM dried bean curds,which was followed by Nantong.With regards to production domains,Chongqing ranked at the first place for the detection rate of GM dried bean curds,with Anhui and Hunan at the second and third places,respectively.This study provided important data analysis and technical support for the supervision of GM foods.

关 键 词：豆干 转基因成分检测 调控元件 功能基因 

分 类 号：TS207.3[轻工技术与工程—食品科学] S565.1[轻工技术与工程—食品科学与工程] Q343.12[农业科学—作物学]