蒸汽蒸馏法对葡萄酒酒精度的快速测定及应用  

Rapid Determination and Application of Wine Alcohol by Steam Distillation

作　　者：刘芳[1] 耿利娜[1] 张建丽[1] 吕雪飞[1] LIU Fang;GENG Lina;ZHANG Jianli;LYU Xuefei(School of Life Science,Beijing Institute of Technology,Beijing 100081,China)

机构地区：[1]北京理工大学生命学院,北京100081

出　　处：《中外葡萄与葡萄酒》2024年第4期111-116,共6页Sino-Overseas Grapevine & Wine

基　　金：教育部产学合作协同育人项目(202102201001,202102439017);北京理工大学开放实验项目(RS202109000049)。

摘　　要：为改善水浴加热蒸馏法的不足,提高葡萄酒酒精度检测效率,借助凯氏定氮仪,基于蒸汽蒸馏法测定葡萄酒酒精度,并进行精密度、回收率试验以及蒸汽蒸馏法和水浴蒸馏法的对比试验,并作测定方法不确定度分析和教学实验应用验证。结果表明,蒸汽蒸馏法对葡萄酒样品酒精度测试值的变异系数均小于水浴蒸馏法;蒸汽蒸馏法可在4 min之内结束蒸馏过程、检测时间缩短一半左右,标准品的乙醇浓度与酒精度测定结果在≤40%体积分数范围内线性关系良好,加标样品酒精度测试标准差0.13~0.45、加标回收率98.55%、RSD均值1.45%,操作简便高效,测试结果准确度高,可推广应用于生产检验或科研教学,并据此结果对葡萄酒酒精度快速测定自动蒸馏仪进行了初步设计与改进。In order to improve the shortcomings of distillation by water bath heating and improve the detection efficiency of alcohol content for wine,the Kjeldahl nitrogen analyzer was introduced to determine the alcohol content of wine based on steam distillation method,and precision and recovery experiments were carried out,as well as a comparative experiment between steam distillation and water bath distillation.The uncertainty analysis was conducted on the measurement methods,and their application and validation in teaching experiments.The results showed that the CV of alcohol content in wine samples tested by steam distillation method was smaller than that of water bath distillation method;through steam heating,the distillation process could be completed within 4 minutes,the detection time could be shortened by about half,and there was a better linear relationship between ethanol concentration and measurement results in the range of≤40%volume fraction,the standard deviation of alcohol content test for samples with added standards ranged from 0.13 to 0.45,the recovery rate was 98.55%,and the average RSD was 1.45%.The steam distillation method was easy to operate,improved detection efficiency,and had good accuracy.It could be applied to related production inspection or research and teaching.Based on the results,a preliminary design and improvement were made on the automatic distillation instrument for rapid determination of wine alcohol content.

关 键 词：蒸汽蒸馏法 水浴蒸馏法 酒精度测定 教学应用 葡萄酒 

分 类 号：TS262.61[轻工技术与工程—发酵工程]