沙棘黄酮通过调控TLR4/NF-κB信号通路改善大鼠多囊卵巢综合征的作用  

Effect of Hippophae rhamnoides Flavone on Improving Polycystic Ovarian Syndrome in Rats by Regulating TLR4/NF-κB Signaling Pathway

作　　者：王玉净 都治香[1] 张霞[1] 王旭[1] 王娜[1] WANG Yujing;DU Zhixiang;ZHANG Xia;WANG Xu;WANG Na(The First Hospital of Hebei Medical University,Shijiazhuang 050030,China)

机构地区：[1]河北医科大学第一医院,河北石家庄050030

出　　处：《食品工业科技》2024年第16期340-347,共8页Science and Technology of Food Industry

基　　金：河北省卫健委指令性课题(20210081)。

摘　　要：目的:探究沙棘黄酮改善大鼠多囊卵巢综合征(polycystic ovarian syndrome,PCOS)的作用及机制,为PCOS的防治提供新的思路。方法:采用高脂饲料联合灌胃来曲唑法复制PCOS模型,并随机分为模型组、低、高剂量沙棘黄酮组(200、400 mg/kg)及二甲双胍组(100 mg/kg),灌胃21 d后检测相关指标变化。结果:与模型组比较,低、高剂量沙棘黄酮组大鼠卵巢指数、发情间期时像百分率、空腹血糖(FBG)、空腹胰岛素(FINS)含量、胰岛素抵抗指数(HOMA-IR)、血清促黄体生成素(LH)活性及睾酮(T)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、C反应蛋白(CRP)含量显著降低(P<0.05,P<0.01),促卵泡刺激素(FSH)活性极显著增加(P<0.01);低、高剂量沙棘黄酮组大鼠卵巢组织病理形态有所改善,Toll样受体4(TLR4)、髓样分化因子88(My D88)、核转录因子κBp65(NF-κBp65)mRNA及TLR4、MyD88、p-NF-κBp65蛋白表达显著降低(P<0.05,P<0.01)。结论:沙棘黄酮可以改善PCOS大鼠症状,减轻胰岛素抵抗,调节性激素水平,修复卵巢组织病理改变,其机制与抑制TLR4/NF-κB信号通路介导的炎症反应有关。Objective:To investigate the effect and mechanism of Hippophae rhamnoides flavone(HRF)on polycystic ovarian syndrome(PCOS)in rats,and to provide new ideas for the prevention and treatment of PCOS.Methods:A PCOS rat model was duplicated by giving rats a high-fat diet combined with the intragastric administration of letrozole,and the model rats were randomly divided into model group,low-dose HRF group(200 mg/kg HRF),high-dose HRF group(400 mg/kg HRF)and metformin group(100 mg/kg).Changes in the related indicators were detected on the 21 d after the rats were administered with the different agents by gavage.Results:Compared with that in the model group,the ovarian index,the percentage of interestrus temporal image,the fasting blood glucose(FBG)level,the fasting insulin(FINS)content,the insulin resistance index(HOMA-IR),the activities of serum luteinizing hormone(LH)and testosterone(T),the levels of tumor necrosis factorα(TNF-α),interleukin 6(IL-6)and C reactive protein(CRP)of rats in low-and high-dose HRF groups were respectively decreased significantly(P<0.05,P<0.01),while the activity of follicle stimulating hormone(FSH)was significantly increased(P<0.01).The histopathological morphology of ovarian tissue of rats treated with the low and high doses of HRF was improved,and the expression levels of toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88)and nuclear factors-κBp65(NF-κBp65)mRNAs as well as TLR4,MyD88 and p-NF-κBp65 proteins were significantly decreased(P<0.05,P<0.01).Conclusion:HRF could improve the symptoms of PCOS,alleviate the insulin resistance,regulate the level of sex hormones and repair the pathological changes in the ovarian tissue of rats with PCOS,and its mechanism may be related to its inhibition on the inflammatory response mediated by the TLR4/NF-κB signaling pathway.

关 键 词：沙棘黄酮 多囊卵巢综合征 炎症反应 Toll样受体4/核转录因子κB信号通路 

分 类 号：TS201.4[轻工技术与工程—食品科学]