黄刺多糖对STZ诱导的Ⅰ型糖尿病大鼠糖脂代谢的调节作用  

Effect of Berberis dasystachya Polysaccharide on Glucose and Lipid Metabolisms in STZ-induced Type I Diabetic Rats

作　　者：马娜娜 韩丽娟 杨永晶 索有瑞[2,3] 院珍珍 叶英 MA Nana;HAN Lijuan;YANG Yongjing;SUO Yourui;YUAN Zhenzhen;YE Ying(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]中国科学院西北高原生物研究所,青海西宁810001 [3]中国科学院大学,北京100049

出　　处：《食品工业科技》2024年第16期348-357,共10页Science and Technology of Food Industry

基　　金：国家自然基金项目(31960088)。

摘　　要：为探究黄刺多糖(Berberis dasystachya Maxim.Polysaccharide,BDP)对链脲佐菌素(STZ)诱导的I型糖尿病大鼠的糖脂代谢的调节作用,本文采用STZ诱导I型糖尿病大鼠模型,糖尿病模型大鼠随机分为模型对照组、多糖低剂量组(BDP-L,100 mg/kg)、中剂量组(BDP-M,200 mg/kg)、高剂量组(BDP-H,400 mg/kg)。灌胃28 d后,监测大鼠体重及血糖变化,测定血脂水平、脂代谢酶活性及体内抗氧化酶活等指标。结果表明:黄刺多糖给药第28 d,与模型组相比BDP给药组的血糖、血脂水平均显著降低(P<0.05),其中高剂量组血糖下降39.16%(P<0.01)。BDP给药组的血清胰岛素水平、肝糖原(HG)水平有所增加(P<0.05或P<0.01),其中高剂量组的大鼠血清胰岛素水平增加1.28倍(P<0.01),HG水平增加89.79%(P<0.01)。相比较模型组大鼠,经BDP给药后大鼠/血清和胰腺中的过氧化氢酶(CAT),超氧化物歧化酶(SOD)和还原型谷胱甘肽(CSHPx)以剂量依赖性方式显著增加(P<0.05或P<0.01),丙二醛(MDA)含量显著减少(P<0.05)。综上,黄刺多糖通过改善Ⅰ型糖尿病大鼠氧化应激从而保护胰岛β细胞的损伤,进而改善糖尿病大鼠的糖脂代谢。In order to provide theoretical support for diabetes prevention and therapy,this study investigated the regulatory effect of Berberis dasystachya Maxim.Polysaccharide(BDP)on glucose and lipid metabolism in streptozotocin(STZ)-induced diabetic rats.Diabetic model rats were randomly assigned to the model control group,low-dose polysaccharide group(BDP-L,10 mg/kg),mid-dose polysaccharide group(BDP-M,200 mg/kg),and high-dose group(BDP-H,400 mg/kg).Blood lipid levels,lipid metabolic enzyme activity,and antioxidant enzyme activity served as evaluation markers.Results showed that after 28 days of administering yellow thorn polysaccharide,the BDP-treated groups exhibited significantly(P<0.05)lower blood glucose and lipid levels compared to the model group,with a remarkable 39.16%reduction in blood glucose in the high-dose group(P<0.01).In contrast,serum insulin levels and hepatic glycogen(HG)levels increased in the BDP-treated groups(P<0.05 or P<0.01),with a 1.28-fold increase in serum insulin(P<0.01)and an 89.79%increase in HG(P<0.01)in the high-dose group.Furthermore,compared to the model group,serum and pancreatic levels of catalase(CAT),superoxide dismutase(SOD),and reduced glutathione(GSH-Px)were significantly elevated(P<0.05 or P<0.01),while malondialdehyde(MDA)levels were considerably reduced(P<0.05)in a dose-dependent manner following BDP treatment.In conclusion,BDP effectively improved glucolipid metabolism in diabetic rats by alleviating oxidative stress,ultimately safeguarding pancreaticβ-cell integrity in type I diabetes.

关 键 词：黄刺多糖 Ⅰ型糖尿病 糖脂代谢 氧化应激 

分 类 号：TS201.4[轻工技术与工程—食品科学]