硒化刺五加多糖对D-半乳糖诱导小鼠氧化损伤的保护作用  

Protective Effects of Selenated Acanthopanax senticosus Polysaccharide Against D-Galactose-induced Oxidative Damage in Mice

作　　者：李晓丽 苏建青[1,2] 李莹 薛娇娇[1] 张瑞 丁一 张学平 冯艺超 王雪艳 褚秀玲 LI Xiaoli;SU Jianqing;LI Ying;XUE Jiaojiao;ZHANG Rui;DING Yi;ZHANG Xueping;FENG Yichao;WANG Xueyan;CHU Xiuling(College of Agronomy and Agricultural Engineering,Liaocheng University,Liaocheng 252000,China;Hebei Veterinary Immunity Enhancer Industry Technology Research Institute,Shijiazhuang 050000,China)

机构地区：[1]聊城大学农学与农业工程学院,山东聊城252000 [2]河北省兽用免疫增强剂产业技术研究院,河北石家庄050000

出　　处：《食品工业科技》2024年第16期368-375,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金(32172901);中央引导地方科技发展资金(216Z6601G);石家庄市后补助奖励专项资金(225790347H)。

摘　　要：为了研究硒化刺五加多糖(Se-ASPS)对D-半乳糖(D-gal)诱导小鼠氧化损伤的保护作用。采用D-gal腹腔注射建立小鼠氧化损伤模型,高、中、低剂量组分别添加硒化刺五加多糖200、100、50 mg/kg·bw,小鼠连续灌胃28 d。比较各组小鼠的体重和采食量变化及脏器指数,采用HE染色观察小鼠肝脏病变。使用试剂盒分析血清、心、肝、脾和肾组织中超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、谷胱甘肽过氧化物酶(Glutathione Peroxidase,GSH-Px)、丙二醛(Malondialdehyde,MDA)、谷草转氨酶(Glutamine Transferase,AST)和谷丙转氨酶(Alglutamine Transferase,ALT)的含量。与D-gal模型组相比,硒化刺五加多糖组均能显著提高小鼠体重(P<0.05),且硒化刺五加多糖高剂量组能显著提高小鼠脏器指数(P<0.05);HE染色发现,硒化刺五加多糖能修复D-半乳糖对小鼠肝脏组织造成的损伤;硒化刺五加多糖高剂量组可显著提高小鼠血清、心、肝、脾、肾组织中SOD、GSH-Px、CAT酶的活性(P<0.05),并显著降低MDA、AST、ALT含量(P<0.05),且高剂量组表现最突出(P<0.01)。Se-ASPS能够明显地保护机体对抗D-gal带来的氧化损伤。To investigate the protective effect of selenated Acanthopanax polysaccharide(Se-ASPS)against D-galactose-induced(D-gal)oxidative damage in mice.The oxidative damage model of mice was established by intrabitoneal injection of D-gal.The mice in the high,medium and low dose groups were supplemented with 200,100 and 50 mg/kg·bw of selenated Acanthopanax polysaccharide,respectively.The mice were gavaged continuous intragastric administration for 28 days.Changes in body weight and feed intake and organ indices of mice in each group were compared,and HE staining was used to observe liver lesions in mice.Serum,heart,liver,spleen and kidney tissues were analyzed for SOD,CAT,GSH-Px,MDA,AST,ALT using the kit.Compared with the D-gal model group,both selenated Acanthopanax polysaccharide groups significantly increased the body weights of mice(P<0.05),and the selenated Acanthopanax polysaccharide high-dose group significantly increased the organ index of mice(P<0.05).HE staining revealed that selenated Acanthopanax polysaccharide could repair the damage caused by D-galactose to mouse liver tissue.The high-dose group of selenated Acanthopanax polysaccharides significantly increased the activities of SOD,GSH-Px,and CAT enzymes in serum,heart,liver,spleen,and kidney tissues of mice(P<0.05),and significantly decreased the contents of MDA,AST,and ALT(P<0.05),with the high-dose group showing the most prominent performance(P<0.01).Se-ASPS can significantly protect the organism against oxidative damage caused by D-gal.

关 键 词：硒化刺五加多糖 D-半乳糖 小鼠 氧化损伤 

分 类 号：TS201.4[轻工技术与工程—食品科学]