穇子总黄酮成分及其对秀丽隐杆线虫的抗衰老作用  

Flavonoid Composition of Finger Millet(Eleusine coracana(L.) Gaertn.) and Its Anti-aging Effect on Caenorhabditis elegans

作　　者：王赛男 陈丹妮 胡克坚 刘嘉敏 李康瑗 王嘉炜 陈立平 王祺皓 肖苏尧[1] WANG Sainan;CHEN Danni;HU Kejian;LIU Jiamin;LI Kangyuan;WANG Jiawei;CHEN Liping;WANG Qihao;XIAO Suyao(Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods,College of Food Science,South China Agricultural University,Guangzhou 510642,China;Key Laboratory of Comprehensive Utilization of Dominant Plants Resources in South Hunan,College of Chemistry and Bioengineering,Hunan University of Science and Engineering,Yongzhou 425199,China)

机构地区：[1]华南农业大学食品学院,广东省功能食品活性物重点实验室,广东广州510642 [2]湖南科技学院化学与生物工程学院,湘南优势植物资源综合利用湖南省重点实验室,湖南永州425199

出　　处：《食品科学》2024年第14期94-102,共9页Food Science

基　　金：国家自然科学基金青年科学基金项目(31100433)。

摘　　要：实验对传统杂粮穇子(Eleusine coracana (L.) Gaertn.)总黄酮进行提取和成分分析,并以秀丽隐杆线虫为模型研究总黄酮的抗衰老活性,为穇子的功能性应用提供理论依据。体积分数70%的乙醇溶液提取、乙酸乙酯萃取和D101大孔树脂纯化得到穇子黄酮(Eleusine coracana flavonoids,ECFs),采用NaNO_(2)-Al(NO_(3))_(3)-NaOH分光光度法测定总黄酮含量,超高效液相色谱-串联质谱进行成分分析,化学法测定体外抗氧化活性,最后以秀丽隐杆线虫为模型系统评价ECFs的抗衰老活性。含量及成分分析结果显示,提取得到的ECFs样品中总黄酮占82.6%,含量较高的成分为16个,除儿茶素外均为黄酮苷类物质;体外抗氧化结果显示,ECFs对1,1-二苯基-2-三硝基苯肼自由基和2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基的半抑制质量浓度(half-maximal inhibiting concentration,IC_(50))分别为45.34μg/mL和22.75μg/mL,其中对ABTS阳离子自由基的IC_(50)甚至优于阳性对照VE(29.25μg/mL)。线虫体内实验结果显示,ECFs质量浓度为200μg/mL时,线虫内过氧化氢酶活力比对照组提高53.98%,超氧化物歧化酶的活力提高30.64%,谷胱甘肽水平提高51.06%,丙二醛的含量降低11.82%,线虫最长寿命延长17.86%;线虫移动力均优于空白组,表明线虫的生命活力得到了提升;线虫产卵量无显著变化,表明ECFs对线虫生殖功能无明显影响;线虫脂褐素水平降低53.91%,表明ECFs处理能有效减少线虫脂褐素沉积。上述结果表明ECFs具有显著的体内外抗氧化效果和线虫抗衰老活性,这为穇子的功能性应用研究提供了基础。In this study,the total flavonoid composition and bioactivity of finger millet was analyzed.Eleusine coracana flavonoids(ECFs) were obtained through sequential 70% ethanol extraction,ethyl acetate extraction,and purification with D101 macroporous resin.The total flavonoid content was determined using the NaNO_(2)-Al(NO_(3))_(3)-NaOH colorimetric method,and the flavonoid composition using ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The in vitro antioxidant activity of ECFs was evaluated using chemical assays,and the anti-aging activity using C.elegans as a model organism.The results demonstrated that the total flavonoid content of ECFs was 82.6%,with 16 major components being identified,including flavonoid glycosides and catechin.In vitro antioxidant tests showed that ECFs exhibited half-maximal inhibitory concentrations(IC_(50)) of 45.34 and 22.75 μg/m L against 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) cation radicals,respectively,with the IC_(50)against ABTS cation radicals being lower than that of the positive control vitamin E(29.25 μg/mL).At a concentration of 200 μg/mL,ECFs increased catalase activity in C.elegans by 53.98%,superoxide dismutase activity by 30.64%,and glutathione levels by 51.06%,while decreasing malondialdehyde(MDA) content by 11.82%.ECFs extended the lifespan of C.elegans by 17.86%,and improved its mobility compared to the control group,indicating improved vitality.ECFs had no significant effect on C.elegans fecundity suggesting no promoting effect on its reproductive function.Additionally,ECFs reduced the level of lipofuscin by 53.91%.These findings demonstrated that ECFs had significant antioxidant effects both in vitro and in vivo as well as remarkable anti-aging activity in C.elegans,which could provide a foundation for the functional application of finger millet.

关 键 词：穇子 总黄酮 秀丽隐杆线虫 抗衰老活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]