基于点击-去点击化学反应的Hg^(2+)荧光探针检测体系构建  

Construction of Fluorescent Probe System Based on Click-Declick Chemical Reaction for Hg^(2+) Detection

作　　者：陶健 成婕 孙志远 尹玉云 杨晶晶 张汝凡 孙朋娟 蔡硕 屈凌波 杨冉[1,3] TAO Jian;CHENG Jie;SUN Zhiyuan;YIN Yuyun;YANG Jingjing;ZHANG Rufan;SUN Pengjuan;CAI Shuo;QU Lingbo;YANG Ran(Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Market Regulation,Zhengzhou 450003,China;Henan Institute of Food and Salt Industry Inspection Technology,Zhengzhou 450003,China;College of Chemistry,Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]国家市场监管重点实验室(食品安全快速检测与智慧监管技术),河南郑州450003 [2]河南省食品和盐业检验技术研究院,河南郑州450003 [3]郑州大学化学学院,河南郑州450000

出　　处：《食品科学》2024年第16期255-261,共7页Food Science

基　　金：国家市场监督管理总局科技计划项目(2021MK066);河南省科技攻关计划项目(222102410011,242102411001);河南省市场监督管理局科技计划项目(2020sj30,2023sj105)。

摘　　要：基于杂化罗丹明与巯基化合物之间的点击化学反应及巯基化合物与金属汞离子的配位反应导致的去点击化学反应机理,构建香豆素杂化罗丹明探针-1,3,5-苯三硫酚-Hg^(2+)的三元荧光检测体系。在该体系中,探针能够与1,3,5-苯三硫酚中的巯基基团发生加成反应,破坏探针的共轭结构,从而导致探针的红色荧光猝灭。加入Hg^(2+)后,Hg^(2+)与1,3,5-苯三硫酚通过配位作用结合,使其从探针结构上离去,探针的共轭结构及红色荧光恢复。体系的荧光强度与Hg^(2+)在5×10^(-9)~1×10^(-8)、1×10^(-8)~1.1×10^(-7)mol/L浓度范围内呈良好的线性关系,检测限为2×10^(-9)mol/L,等倍量的常见重金属离子对Hg^(2+)的特异性识别无干扰。应用于实际饮用水样品中Hg^(2+)含量检测,回收率可达到86.7%~110.2%。Based on the click chemical reaction between hybrid rhodamine and thiol compounds and the declick chemical reaction caused by the coordinative interaction between thiol compounds and mercury ions,a ternary system of coumarin hybrid rhodamine probe,1,3,5-benzenetrithiol and Hg^(2+)for fluorescence detection of Hg^(2+)was constructed.In this system,the probe underwent an addition reaction with the thiol group in 1,3,5-benzenetrithiol,destroying the conjugate structure of the probe and thus resulting in quenching of its red fluorescence.Hg^(2+)could remove 1,3,5-benzenetrithiol from the probe’s structure by coordinative binding to the compound,so the conjugate structure and red fluorescence of the probe were restored.The fluorescence intensity of the probe had a good linear relationship with the concentration of Hg^(2+)in the range of 5×10-9-1×10_(-8) and 1×10^(-8)–1.1×10^(-7) mol/L,and the detection limit was 2×10^(-9) mol/L.Common heavy metal ions had no significant interference on the specific recognition of Hg^(2+).The recoveries of Hg^(2+)from spiked drinking water samples ranged from 86.7%to 110.2%.

关 键 词：三元荧光检测体系 荧光探针 Hg^(2+) 快速检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]