Pin1和paxillin蛋白在肺癌组织中的表达及临床意义  

Expressions of Pin1 and paxillin proteins in lung cancer tissues and their clinical significance

作　　者：李娜 刘存宇[1] 何志巍 LI Na;LIU Cunyu;HE Zhiwei(China-America Cancer Research Institute,Guangdong Medical University,Dongguan 523808,China;Department of Pathology,the Second Affiliated Hospital of Guangzhou Medical University,Guangdong 510260,China)

机构地区：[1]广东医科大学中美肿瘤研究所,东莞523808 [2]广州医科大学附属第二医院病理科,广东510260

出　　处：《中国临床新医学》2024年第7期721-727,共7页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：国家自然科学基金面上项目(编号:81372137)。

摘　　要：目的探讨Pin1和paxillin蛋白在肺癌组织及癌旁正常肺组织中的表达差异,并分析二者与临床病理特征的相关性。方法收集2018年6月至2022年9月广州医科大学附属第二医院收治的208例原发性肺癌患者的病历资料和病理组织,采用免疫组化染色(SP法)检测组织中Pin1和paxillin的表达,分析其与临床病理特征的关系。Western blot检测多个肺癌细胞系细胞中Pin1和paxillin蛋白表达水平。应用Kaplan-Meier生存曲线评估Pin1和paxillin表达与患者预后的关系。结果肺癌组织中Pin1和paxillin的表达水平高于癌旁正常肺组织,差异有统计学意义(P<0.05)。Pin1蛋白表达与T分期、淋巴结转移和临床分期具有相关性(P<0.05);paxillin蛋白表达与组织学类型、淋巴结转移具有相关性(P<0.05)。Spearman秩相关分析结果显示,肺癌组织中Pin1与paxillin蛋白表达呈正相关(r_(s)=0.455,P<0.001)。Western blot结果显示,Pin1和paxillin在不同肺癌细胞株中表达高低不一。Paxillin低表达组患者的生存预后显著优于paxillin高表达组(P<0.05),Pin1表达水平则与肺癌患者生存预后无显著关联(P>0.05)。结论肺癌组织中Pin1和paxillin蛋白表达升高,与临床病理特征相关。肺癌组织中Pin1与paxillin表达呈正相关,且paxillin表达水平可作为肺癌患者预后的重要判断指标之一。Objective To explore the differences in the expressions of Pin1 and paxillin proteins in lung cancer tissues and paracancerous normal tissues,and to analyze their correlations with clinicopathological features.Methods The medical record data and pathological tissues of 208 patients with primary lung cancer who were admitted the Second Affiliated Hospital of Guangzhou Medical University from June 2018 to September 2022 were collected.Immunohistochemical staining(SP method)was used to detect the expressions of Pin1 and paxillin in the tissues,and their correlations with clinicopathological features was analyzed.Western blot was used to detect the expression levels of Pin1 and paxillin proteins in multiple lung cancer cell line cells.Kaplan-Meier survival curve was used to evaluate the correlations of Pin1 and paxillin expressions with the patients′prognosis.Results The levels of Pin1 and paxillin expressions in lung cancer tissues were higher than those in paracancerous normal tissues,and the differences were statistically significant(P<0.05).The expression of Pin1 protein was correlated with T stage,lymph node metastasis and clinical stage(P<0.05),and the expression of paxillin protein was correlated with histological type and lymph node metastasis(P<0.05).The results of Spearman rank correlation analysis showed a positive correlation between Pin1 protein expression and paxillin protein expression in lung cancer tissues(r_(s)=0.455,P<0.001).The results of Western blot showed that the expressions of Pin1 and paxillin varied in different lung cancer cell lines.The survival prognosis of the patients in the paxillin low expression group was significantly better than that of the patients in paxillin high expression group(P<0.05),and the level of Pin1 expression was not significantly correlated with the survival prognosis of the lung cancer patients(P>0.05).Conclusion The expressions of Pin1 and paxillin proteins are increased in lung cancer tissues,which are correlated with clinicopathological features.There is a pos

关 键 词：肺癌 PIN1 PAXILLIN 预后 相关性 

分 类 号：R734.2[医药卫生—肿瘤]