全基因组测序分析山东省胶东地区禽源弯曲菌基因组特征和耐药性  

Whole Genome Sequencing Analysis on Genomic Characteristics and Antimicrobial Resistance of Avian Campylobacter in Jiaodong Region,Shandong Province

作　　者：王娟[1] 宋时萍 黄秀梅[1] 刘俊辉[1] 王琳 苗帅 刘娜[1] 赵建梅[1] 高玉斌 赵格[1] 张喜悦[1] 王君玮[1] 曲志娜[1] Wang Juan;Song Shiping;Huang Xiumei;Liu Junhui;Wang Lin;Miao Shuai;Liu Na;Zhao Jianmei;Gao Yubin;Zhao Ge;Zhang Xiyue;Wang Junwei;Qu Zhina(China Animal Health and Epidemiology Center,Qingdao Key Laboratory of Modern Bioengineering and Animal Disease Research,Qingdaoo 266032,Shandong,China;Northeast Agricultural University,Harbin 150006,Heilongjiang,China)

机构地区：[1]中国动物卫生与流行病学中心,青岛市现代生物工程及动物疫病研究重点实验室,山东青岛266032 [2]东北农业大学,黑龙江哈尔滨150006

出　　处：《中国动物检疫》2024年第7期22-28,共7页China Animal Health Inspection

基　　金：国家重点研发计划项目(2022YFC2303900)。

摘　　要：为了解山东省胶东地区禽源弯曲菌的耐药特征、毒力特征及分型遗传进化关系,对2021-2022年来自胶东地区26个养禽场的31株弯曲菌,采用肉汤稀释法进行抗生素敏感性检测,然后基于全基因组测序结果分析其耐药基因、毒力基因、多位点序列分型,并进行全基因组单核苷酸多态性(wgSNPs)遗传进化分析。结果显示:31株弯曲菌对四环素的耐药率为96.77%,对环丙沙星、萘啶酸的耐药率均为93.55%;31株菌呈现出6种耐药谱,15株菌耐3类及以上药物。基于全基因组测序结果,31株弯曲菌分为20个序列型(ST),其中结肠弯曲菌优势克隆群为CC828,空肠弯曲菌呈现多样性分布;携带喹诺酮类、四环素类、氨基糖苷类、β-内酰胺类等多种类型耐药基因,携带已知功能的6类42种毒力基因。经wgSNPs遗传进化分析,结肠弯曲菌优势克隆群CC828分布在3个小分支中,空肠弯曲菌分布在7个小分支中,分布分散。结果表明:胶东地区禽源弯曲菌耐药严重,耐药谱较广,携带的耐药基因与耐药表型有一定关系;携带毒力基因数量较多,结肠弯曲菌有优势克隆群,空肠弯曲菌ST型呈多样性,分离菌株具有较高的遗传多样性。本研究为降低禽源弯曲菌向人类传播的概率,实现“同一健康”提供了技术支撑。In order to identify the drug resistance,virulence and genotype genetic evolution of avian Campylobacter in Jiaodong region of Shandong Province,31 strains of Campylobacter isolated from 26 poultry farms in the region from 2021 to 2022 were tested for antibiotic susceptibility using broth dilution method,and their resistance genes,virulence genes,and multilocus sequence typing were analyzed based on the results of whole genome sequencing(WGS),followed by genetic evolutionary analysis on whole genome single nucleotide polymorphisms(wgSNPs).The results showed that the 96.77%of 31 strains were resistant to tetracycline,93.55%resistant to ciprofloxacin and nalidixic acid;for the 31 strains of Campylobacter,6 resistance spectrums were obtained and 15 strains were resistant to 3 or more drugs.WGS results showed that the 31 strains could be classified into 20 sequence types(STs),specifically,the dominant clonal group of Campylobacter colonis(C.colonis)was CC828,while Campylobacter jejuni(C.jejuni)showed diversity;antibiotic resistance genes associated with quinolones,tetracycline,aminoglycosides andβ-lactam were found,42 virulence genes of 6 classes with known functions were carried.The wgSNPs phylogenetic analysis indicated that the dominant clonal group CC828 was distributed in 3 sub-clades,and C.jejuni was dispersedly in 7 subclades.In conclusion,the antimicrobial resistance of avian Campylobacter was relatively severe in Jiaodong region,with a wide range of drug resistance spectrums,and the drug-resistant genes carried were related to the drug-resistant phenotypes to certain extent;a large amount of virulence genes were carried,a dominant clonal group of C.colonis was found,ST-type of C.jejuni was various,and the isolated strains were genetically diverse.A technical support was provided for reducing the probability of transmission of avian Campylobacter to humans under the concept of“One Health”.

关 键 词：禽源弯曲菌 全基因组测序 耐药性 毒力特征 

分 类 号：S852.43[农业科学—基础兽医学]